Abstract
In order to achieve expression of a foreign gene in a bacterial cell it is necessary to put that gene under the control of the transcriptional and translational machinery of the host cell. The bacterium E. coli is a natural choice for such studies because of our detailed knowledge of the molecular biology of its gene expression. This chapter will examine the suitability of the most commonly used cloning vehicles as systems for expressing foreign genes.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Clarke, L. and Carbon, J. (1975) Proc. natn Acad. Sci. USA. 72, 4361.
Clarke, L. and Carbon, J. (1976) Cell, 9, 91.
Struhl, K., Cameron, J. R. and Davis, R. W. (1976), Proc. natn Acad. Sci. USA. 73, 1471.
Ratzkin, B. and Carbon, J. (1977) Proc. natn Acad. Sci. USA. 74, 487.
Rambach, A. and Hogness, D. S. (1978) Proc. natn Acad. Sci. USA. 74, 5041.
Broome, S. and Gilbert, W. (1978) Proc. natn Acad. Sci. USA. 75, 2746.
Erlich, H. A., Cohen, S. N. and McDevitt, H. O. (1978) Cell 13, 681.
Alwine, J. C., Kemp, D. J. and Stark, G. R. (1977) Proc. natn Acad. Sci. USA. 74, 5350.
Itakura, K., Hirose, T., Crea, R., Riggs, A. D., Heyneker, H. L., Bolivar, F. and Boyer, H. W. (1977) Science 198, 1056.
Maniatis, T., Kee, S. E., Efstratiadis, A. and Kafatos, F. (1976) Cell 8, 163.
Villa-Komaroff, L., Efstratiadis, A., Broome, S., Lornedico, P., Tizard, R., Naber, S. P., Chick, W. L. and Gilbert, W. (1978) Proc. natn Acad. Sci. USA. 75, 3727.
Seeburg, P. H., Shine, J., Martial, J. A., Ivarie, R. D., Morris, J. A., Ullrich, A., Baxter, J. D. and Goodman, H. M. (1978) Nature 276, 795.
Burrell, C. J., Mackay, P., Greenaway, P. J., Hofschneider, P. and Murray, K. (1979) Nature 279, 43.
Chang, A. C. Y., Nunberg, J. H., Kaufman, R. J., Erlich, H. A., Schimke, R. T. and Cohen, S. N. (1978) Nature 275, 617.
Shine, J. and Dalgano, L. (1974) Proc. natn Acad. Sci. 71, 1342.
Nagata, S., Taira, H., Hall, A., Johnsrud, L., Streuli, M., Ecsodi, J., Boll, W., Cantell, K. and Weissmann, C. (1980), Nature, 284, 316.
Stewart, W. E. (1979) ‘The Interferon System’ Springer Verlag, Wien and New York.
Goeddel, D. V., Heyneker, H. L., Hozumi, T., Arentzen, R., Itakura, K., Yansura, D. G., Ross, K. J., Miozzari, G., Crea, R., Seeburg, P. H. (1979) Nature 281, 544.
Roberts, T. M., Bikel, I., Rogers Yocum, R., Livingstone, D. M., Ptashne, M. (1979) Proc. natn Acad. Sci. USA 76, 5596.
Murray, N. E., Bruce, S. and Murray, K. (1979). J. mol. Biol. 132, 493.
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1980 D. M. Glover
About this chapter
Cite this chapter
Glover, D.M. (1980). Expression of cloned DNAs in E. coli. In: Genetic Engineering Cloning DNA. Genetic Engineering: Principles and Methods. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-7646-8_5
Download citation
DOI: https://doi.org/10.1007/978-94-015-7646-8_5
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-412-16170-4
Online ISBN: 978-94-015-7646-8
eBook Packages: Springer Book Archive