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Bacteriophage λ vectors

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Genetic Engineering Cloning DNA

Part of the book series: Genetic Engineering: Principles and Methods ((GEPM))

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Abstract

Bacteriophage λ has about 50 genes in its 49Kb genome, only about half of which are essential for its lytic growth. Before describing λ vectors in which this non-essential DNA can be replaced by foreign DNA, I will give a simplified description of the life cycle of the bacteriophage. More comprehensive reviews can be found elsewhere [1,2].

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References

  1. Hershey, A. D., ed (1971), ‘The Bacteriophage Lambda’ Cold Spring Harbor Laboratory, New York (1971).

    Google Scholar 

  2. ‘Gene Expression III’ (1977), B. Lewin. John Wiley.

    Google Scholar 

  3. Wu, R. and Taylor, E. (1971), J. mol. Biol 57, 491.

    Article  CAS  Google Scholar 

  4. Inman, R. B. (1966), J. mol Biol. 18, 464.

    Article  CAS  Google Scholar 

  5. Bastia, D., Sneoka, N. and Cos, E. C. (1975), J. mol. Biol. 98, 305.

    Article  CAS  Google Scholar 

  6. Enquist, L. W. and Skalka, A. (1973), J. mol. Biol. 75, 185.

    Article  CAS  Google Scholar 

  7. Wang, J. C. and Kaiser, A. D. (1973), Nat. N. B. 241, 16.

    Article  CAS  Google Scholar 

  8. Roberts, J. (1969), Nature 224, 1168.

    Article  CAS  Google Scholar 

  9. Adhya, S., Gottesman, M. and De Crombrugghe, B. (1974), Proc. natn Acad. Sci. USA. 71, 2534.

    Article  CAS  Google Scholar 

  10. Franklin, N. (1974), J. mol. Biol. 89, 33.

    Article  CAS  Google Scholar 

  11. Ptashne, M., Backman, K., Humaynn, M. Z., Jeffrey, A., Maurer, R., Meyer, B. and Sauer, R. T. (1976), Science 194, 156.

    Article  CAS  Google Scholar 

  12. Roberts, J. W., Roberts, C. W. and Mount, D. W. (1977), Proc. natn Acad. Sci. 74, 2283.

    Article  CAS  Google Scholar 

  13. Simon, M. N., Davis, R. W. and Davidson, N. (1971), In ‘The Bacterio-phage Lambda’ (Hershey, A. D., ed.), Cold Spring Harbor Laboratory, New York.

    Google Scholar 

  14. Rambach, A. and Tiollais, P. (1974), Proc. natn Acad. Sci. USA. 71, 3927.

    Article  CAS  Google Scholar 

  15. Murray, N. E. and Murray, K. (1975), Nature 251, 476.

    Article  Google Scholar 

  16. Thomas, M., Cameron, J. R. and Davis, R. W. (1974), Proc. natn Acad. Sci. USA. 71, 4579.

    Article  CAS  Google Scholar 

  17. Murray, N. E., Brammar, W. J. and Murray, K. (1977), Mol. gen. Genet. 150, 53.

    Article  CAS  Google Scholar 

  18. Blattner, F. R., Williams, B. G., Blechl, A. E., Denniston-Thompson, K., Faber, M. E., Furlong, L. A., Grunwald, D. J., Kiefer, D. O., Moore, D. D., Schumm, J. W., Sheldon, E. L. and Smithies, O. (1977), Science 196, 161.

    Article  CAS  Google Scholar 

  19. Hohn, T., Wurtz, M. and Hohn, B. (1976), Phil. Trans. R. Soc. 276, 51.

    Article  CAS  Google Scholar 

  20. Casjens, S. and King, J. (1975), Ann. Rev. Biochem. p. 555.

    Google Scholar 

  21. Leder, P., Tiemeier, D. and Enquist, L. (1977), Science 196, 175.

    Article  CAS  Google Scholar 

  22. Mandel, M. and Higa, A. (1970), J. mol. Biol. 53, 159.

    Article  CAS  Google Scholar 

  23. Hohn, B. and Murray, K. (1977), Proc. natn. Acad. Sci. USA. 74, 3259.

    Article  CAS  Google Scholar 

  24. Sternberg, N., Teimeier, D. and Enquist, L. (1977), Gene 1, 255.

    Article  CAS  Google Scholar 

  25. Maniatis, T., Hardison, R. C., Lacy, E., Lauer, J., O’Connel, C., Quon, D., Sim, G. K. and Efstratiadis, A. (1968), Cell 15, 687.

    Article  Google Scholar 

  26. Benton, W. and Davis, R. W. (1977), Science 196, 180.

    Article  CAS  Google Scholar 

  27. Collins, J. and Bruning, H. J. (1978), Gene 4, 85.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Cancer Research Campaign Eukaryotic Molecular Genetics Group, Department of Biochemistry, Imperial College of Science and Technology, London, UK

    David M. Glover

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  1. David M. Glover
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© 1980 D. M. Glover

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Glover, D.M. (1980). Bacteriophage λ vectors. In: Genetic Engineering Cloning DNA. Genetic Engineering: Principles and Methods. Springer, Dordrecht. https://doi.org/10.1007/978-94-015-7646-8_4

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  • DOI: https://doi.org/10.1007/978-94-015-7646-8_4

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-412-16170-4

  • Online ISBN: 978-94-015-7646-8

  • eBook Packages: Springer Book Archive

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