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Ultraviolet and Fluorescence Microscopy

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Book cover Applied Polymer Light Microscopy

Abstract

The optical system of any microscope can be divided into two main groups or components, the illuminating optics and the observing optics. The two systems are designed so that, in the absence of a sample, the field of view imaged by the observing optics appears uniformly illuminated (or uniformly dark). When a sample is introduced into the microscope, image contrast arises because different regions of the sample affect the illumination to differing extents. The image may result from many effects, including diffraction, refraction, reflection, scattering, interference, polarization, fluorescence and absorption, and microscopes have been designed to take advantage of most of these phenomena. In this chapter we shall be concerned with optical microscopy in which the illuminating light is in the blue/ultraviolet range, from around 230 nm to 400 nm. In this range the main intentional sources of image contrast are UV absorption and fluorescence emission, although other mechanisms, notably diffraction, may cause problems.

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Author information

Authors and Affiliations

  1. School of Chemistry and Molecular Science, University of Sussex, Brighton, UK

    P. Calvert & N. C. Billingham

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  1. P. Calvert
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  2. N. C. Billingham
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Editor information

Editors and Affiliations

  1. Polymer Microscopy Services, 52 Springfield Close, Burton on the Wolds, Loughborough, Leicestershire, LE12 5AN, UK

    D. A. Hemsley

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© 1989 Elsevier Science Publishers Ltd

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Calvert, P., Billingham, N.C. (1989). Ultraviolet and Fluorescence Microscopy. In: Hemsley, D.A. (eds) Applied Polymer Light Microscopy. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-7474-9_7

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  • DOI: https://doi.org/10.1007/978-94-011-7474-9_7

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-011-7476-3

  • Online ISBN: 978-94-011-7474-9

  • eBook Packages: Springer Book Archive

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