Abstract
The use of microorganisms to determine or assess a variety of biologically active substances either qualitatively or quantitatively is well established. Among the earliest applications of microbiological assays were those for vitamins and later for amino acids. During the early 1940s, microorganisms were employed to determine the presence and quantity of many antimicrobial agents, and more recently, to assess the mutagenicity of chemicals. Although other methods have largely supplanted microbiological assays for amino acids and to some extent antibiotics, the microbiological methods continue to be of value, for unlike chemical and physical methods, microbiological assays enable one to assess the biological activity of the agent or compound in question.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
Bibliography
ATCC (American Type Culture Collection). 1978. Catalogue of Strains I, 13th ed. Washington, DC.
AMES, B.N., Mccann, J., and Yamasaki, E. 1975. Methods for detecting carcinogens and mutagene with the Salmonella/mammalian-microsome mutagenicity test. Mutation Res. 31, 347–364.
ANALYTICAL MICROBIOLOGY, II. 1972. F. Kavanagh (Editor). Academic Press, New York.
ANDERSON, M.E., and Williams, H.H. 1951. Microbiological evaluation of protein quality. I. A colorimetric method for the determination of the growth of Tetrahymena geleii W in protein suspensions. J. Nutr. 44, 335–343.
AOAC (Association of Official Analytical Chemists). 1980. Official Methods of Analysis, 13th ed. Washington, DC.
AOAC (Association of Official Analytical Chemists). 1982. Changes in methods. J. Assoc. Off. Anal. Chem. 65, 450–521.
BAKER, H., and Frank, O. 1968. Clinical Vitaminology — Methods and Interpretation. Interscience Publishers, New York.
BAKER, H., Frank, O., Russoff, I.I., Morck, R.A., and Hutner, S.H. 1978. Protein quality of foodstuffs determined with Tetrahymena thermophila and rat. Nutr. Rep. Int. 17, 525–536.
BERG, T.M., and Behagel, H.A. 1972. Semiautomated method for microbiological vitamin assays. Appl. Microbiol. 23; 531–542.
BOLINDER, A.E. 1972. Large plate assays for amino acids, pp. 479–591. In Analytical Microbiology. F. Kavanagh (Editor). Academic Press, New York.
BOYNE, A.W., Price, S.A., Rosen, G.D., and Stott, J.A. 1967. Protein quality of feeding-stuffs. 4. Progress report of collaborative studies on the microbiological assay of available amino acids. Brit. J. Nutr. 21, 181–206.
BRAEKKAN, O.R. 1960. Effect of temperature on a lag observed in the microbiological assay of pantothenic acid. J. Bacteriol. 80, 626–627.
BUCHANAN, R.A. 1969. In vivo and in vitro methods of measuring nutritive values of leaf-protein preparations. Brit. J. Nutr. 23, 533–545.
BUNYAN, J., and Woodham, A.A. 1964. Protein quality of feeding-stuffs. 2. The comparative assessment of protein quality in three fish meals by microbiological and other laboratory tests, and by biological evaluation with chicks and rats. Brit. J. Nutr. 18, 537–544.
BUTRUM, R.R., and Kramer, A. 1977. Comparison of rat and Tetrahymena pyriformis growth assays in determining nutritional quality of protein in meat and meat analogs during prolonged storage at various temperatures. J. Food Qual. 1, 379–391.
CASCIANO, D.A. 1982. Mutagenesis assay methods. Food Technol. 36(3), 48–52.
CHEN, M.F., Mcintyre, P.A., and Kertcher, J.A. 1978. Measurement of folates in human plasma and erythrocytes by a radiometric microbiologic method. J. Nucl. Med. 19, 906–912.
COMMONER, B., Vithayathil, A., and Dolara, P. 1978. Mutagenic analysis as a means of detecting carcinogens in foods. J. Food Protect. 41, 996–1003.
COOPERMAN, J.M. 1967. Microbiological assay of serum and whole-blood folic acid activity. Am. J. Clin. Nutr. 20, 1015–1024.
CRAIN, P.R. 1980. Fundamental aspects of monitoring food related microbes by impedance. Diss. Abstr. Int. B 41(1), 121.
DEBLANC, H.J. Jr., Charache, P., and Wagner, H.N. Jr. 1972. Automatic radiometric measurement of antibiotic effect on bacterial growth. Antimicrobiol. Agents Chemo. 2, 360–366.
DESERRES, F.J., and Shelby, M.D. 1979. The Salmonella mutagenicity assay: Recommendations. Science 203, 563–565.
DIFCO SUPPL. LIT. 1972. Difco Laboratories, Detroit, MI.
DUNN, M.S., and Rockland, L.B. 1947. Biological value of proteins determined with Tetrahymena geleii H. Proc. Soc. Exp. Biol. Med. 64, 377–379.
ELLIOTT, A.M. 1959. Biology of Tetrahymena. Ann. Rev. Microbiol. 13, 79–96.
EVANCHO, G.M., Hurt, H.D., Devlin, P.A., Landers, R.E., and Ashton, D.H. 1977. Comparison of Tetrahymena pyriformis W and rat bioassays for the determination of protein quality. J. Food Sci. 42, 444–448.
EVANS, E., Carruthers, S.C., and Witty, R. 1977. Comparison of Tetrahymena pyriformis and PER responses to meats and meat products. Nutr. Rept. Int. 16, 445–462.
EVANS, E., Khouw, B.T., Likuski, H.J., and Witty, R. 1979A. Effects of altering carbohydrate and buffer on the growth of Tetrahymena pyriformis W and on the determination of the relative nutritive value of food proteins. Can. Inst. Food Sci. Technol. J. 12, 36–39.
EVANS, E., Carruthers, S.C., and Witty, R. 1979B. Effects of cooking methods on the protein quality of meats as determined using a Tetrahymena pyriformis W growth assay. J. Food Sci. 44, 1678–1680.
FERNELL, W.R. and Rosen, G.D. 1956. Microbiological evaluation of protein quality with Tetrahymena pyriformis W. 1. Characteristics of growth of the organism and determination of relative nutritional values of intact proteins. Brit. J. Nutr. 10, 143–156.
FERRARI, A., and Marten, J. 1972. Automated microbiological assay. Methods in Microbiol. 6B, 331–342.
FORD, J.E. 1962. A microbiological method for assessing the nutritional value of proteins. 2. The measurement of “available” methionine, leucine, isoleucine, arginine, histidine, tryptophan and valine. Brit. J. Nutr. 16, 409–425.
FRANK, O., Baker, H., and Hutner, S.H. 1975. Evaluation of protein quality with the phagotrophic protozoan Tetrahymena, pp. 203-209. In Protein Nutritional Quality of Foods and Feeds. M. Friedman (Editor). Marcel Dekker, New York.
GORIN, N., Meulenhoff, E.J.S., and Yarrow, D. 1970. Determination of niacin in orange juice with lyophilized Lactobacillus arabinosus ATCC 8014. Appl. Microbiol. 20, 641–542.
GRAHAM, D.C., Roe, D.A., and Ostertag, S.G. 1980. Radiometric determination and chick bioassay of folacin in fortified and unfortified frozen foods. J. Food Sci. 45, 47–51.
GREGORY, J.F., III. 1980. Comparison of high-performance liquid Chromatographie and Saccharomyces uvarum methods for the determination of vitamin B6 in fortified breakfast cereals. J. Agric. Food Chem. 28, 486–489.
GREGORY, J.F., III. 1983. Methods of vitamin assay for nutritional evaluation of food processing. Food Technol. 37(1), 75–80.
GREGORY, J.F., III, Day, B.P.F., and Ristow, K.A. 1982. Comparison of high performance liquid Chromatographie, radiometric, and Lactobacillus casei methods for the determination of folacin in selected foods. J. Food Sci. 47, 1568–1571.
GUILARTE, T.R., McIntyre, P.A., and Tsan, M.-F. 1980. Growth response of the yeasts Saccharomyces uvarum and Kloeckera brevis to the free biological active forms of vitamin B-6. J. Nutr. 110, 954–958.
GUILARTE, T.R., Shane, B., and McIntyre, P.A. 1981. Radiometric-microbiological assay of vitamin B-6: Application to food analysis. J. Nutr. 111, 1869–1875.
GUIRARD, B.M., and Snell, E.E. 1981. Biochemical factors in growth, pp. 79–111. In Manual of Methods for General Bacteriology. P. Gerhardt et al. (Editors). Am. Soc. Microbiol., Washington, D.C.
HALEVY, S., and Grossowicz, N. 1953. A microbiological approach to nutritional evaluation of proteins. Proc. Soc. Exp. Biol. Med. 82, 567–571.
HANDBOOK OF MICROBIOLOGY, III. 1973. pp. 1017-1051. A.I. Laskin and H.A. Lechevalier (Editors). CRC Press, Cleveland, OH.
HARPER, A.E. 1981. McCollum and directions in the evaluation of protein quality. J. Agric. Food Chem. 29, 429–435.
HEGSTED, D.M. 1977. Protein quality and its determination, pp. 347-362. In Food Proteins. J.R. Whitaker and S. Tannenbaum (Editors). AVI Publishing Co., Westport, CT.
HEGSTED, D.M., and Chang, U.-Y. 1965. Protein utilization in growing rats. I. Relative growth index as a bioassay procedure. J. Nutr. 85, 159–168.
HERBERT, V. 1966. Aseptic addition method for Lactobacillus casei assay of folate activity in human serum. J. Clin. Path. 19, 12–16.
HOPPNER, K. 1971. Free and total folate activity in strained baby foods. Can. Inst. Food Technol. J. 4, 51–54.
HOPPNER, K., Lampi, B., and Perrin, D.E. 1973. Folacin activity of frozen convenience foods. J. Am. Diet. Assoc. 63, 536–539.
ITAGAKI, T., and Tsukahara, T. 1972. Microbiological assay of vitamin B6 by thinlayer cup-plate method with Saccharomyces carlsbergensis. J. Vitaminol. 18, 90–96.
KIDDER, G.W., and Dewey, V.C. 1945. Studies on the biochemistry of Tetrahymena. III. Strain differences. Physiol. Zool. 18, 136–157.
KLEIN, B.P., and Kuo, C.H.Y. 1981. Comparison of microbiological and radiometric assays for determining total folacin in spinach. J. Food Sci. 46, 552–554.
LANDERS, R.E. 1975. Relationship between protein efficiency ratio of foods and relative nutritive value measured by Tetrahymena pyriformis W bioassay techniques, pp. 185-202. In Protein Nutritional Quality of Foods and Feeds. M. Friedman (Editor). Marcel Dekker, New York.
LEICHTER, J., Butterworth, C.E., and Krumdieck, C.L. 1977. Partial purification and some properties of pteroylglutamate hydrolyase (conjugase) from chicken pancreas. Proc. Soc. Exp. Biol. Med. 154, 98–101.
LI-CHAN, E., and Nakai, S. 1981. Nutritional evaluation of covalently lysine enriched wheat gluten by Tetrahymena bioassay. J. Food Sci. 46, 1840–1841, 1850.
MACIEJEWICZ-RYS, J., and Antoniewicz, A.M. 1978. 2-Aminoethylphosphonic acid as an indicator of Tetrahymena pyriformis W growth in protein-quality evaluation assay. Brit. J. Nutr. 40, 83–90.
MANUAL OF METHODS FOR GENERAL BACTERIOLOGY. 1981. P. Gerhardt et al. (Editors). Amer. Soc. Microbiol., Washington, D.C.
McCANN, J., Choi, E., Yamasaki, E., and Ames, B.N. 1975. Detection of carcinogens as mutagene in the Salmonella/microsome test: Assay of 300 chemicals. Proc. Natl. Acad. Sci. 72, 5135–5139.
McCURDY, S.M., Scheier, G.E., and Jacobson, M. 1978. Evaluation of protein quality of five varieties of lentils using Tetrahymena pyriformis W. J. Food Sei. 43, 694–697.
McMARTIN, K.E., Virayotha, V., and Tephly, T.R. 1981. High-pressure liquid chromatography separation and determination of rat liver folates. Arch. Biochem. Biophys. 209, 127–130.
MOHYUDDIN, M., Sharma, T.R., and Niemann, E.-G. 1977. Nutritive value of rye and wheat breads assessed with Aspergillus flavus. J. Agric. Food Chem. 25, 200–201.
NASHEF, A.S., Osuga, D.T., Lee, H.S., Ahmed, A.I., Whitaker, J.R., and Feeney, R.E. 1977. Effects of alkali on proteins. Disulfides and their products. J. Agric. Food Chem. 25, 245–251.
PARIZA, M.W. 1983. Carcinogenicity/toxicity testing and the safety of foods. Food Technol. 37(1), 84–86.
PELLETT, P.L. 1978. Protein quality evaluation revisited. Food Technol. 32(5), 60–79.
PHILLIPS, D.R., and Wright, A.J.A. 1982. Studies on the response of Lactobacillus casei to different folate monoglutamates. Brit. J. Nutr. 47, 183–189.
PHILLIPS, D.R., and Wright, A.J.A. 1983. Studies on the response of Lactobacillus casei to folate vitamin in foods. Brit. J. Nutr. 49, 181–186.
PILCHER, H.L., and Williams, H.H. 1954. Microbiological evaluation of protein quality. II. Studies of the responses of Tetrahymena pyriformis W to intact proteins. J. Nutr. 53, 589–599.
ROCKLAND, L.B., and Dunn, M.S. 1949. Determination of the biological value of proteins with Tetrahymena geleii H. Food Technol. 3, 289–292.
ROSEN, G.D., and Fernell, W.R. 1956. Microbiological evaluation of protein quality with Tetrahymena pyriformis W. 2. Relative nutritive values of protein in foodstuffs. Brit. J. Nutr. 10, 156–169.
ROTHENBERG, S.P., Dacosta, M., and Rosenberg, Z. 1972. A radioassay for serum folate: Use of a two-phase sequential-incubation, ligand-binding assay. New Eng. J. Med. 286, 1335–1339.
RUDDICK, J.E., Vanderstoep, J., and Richards, J.F. 1978. Folate levels in food — A comparison of microbiological assay and radioassay methods for measuring folate. J. Food Sci. 43, 1238–1241.
SCHATZKI, T.F., and Keagy, P.M. 1975. Analysis of nonlinear response in microbiological assay for folacin. Anal. Biochem. 65, 204–214.
SHANE, B., Tamura, T., and Stokstad, E.L.R. 1980. Folate assay: A comparison of radioassay and microbiological methods. Clin. Chem. Acta 100, 13–19.
SHEPHERD, N.D., Taylor, T.G., and Wilton, D.C. 1977. An improved method for the microbiological assay of available amino acids in proteins using Tetrahymena pyriformis. Brit. J. Nutr. 38, 245–253.
SHORROCK, C. 1976. An improved procedure for the assay of available lysine and methionine in feedstuffs using Tetrahymena pyriformis W. Brit. J. Nutr. 35, 333–341.
SMITH, E.B., and Pena, P.M. 1977. Use of Tetrahymena pyriformis W to evaluate protein quality of leaf protein concentrates. J. Food Sci. 42, 674–676.
SNELL, E.E. 1948. Use of microorganisms for assay of vitamins. Physiol. Rev. 28, 255–282.
SRINIVAS, H., Vakil, U.K., and Sreenivasan, A. 1975. Evaluation of protein quality of irradiated foods using Tetrahymena pyriformis W and rat assay. J. Food Sei. 40, 66–69.
STERNBERG, M., and Kim, C.Y. 1979. Growth response of mice and Tetrahymena pyriformis to lysinoalanine-supplemented wheat gluten. J. Agric. Food Chem. 27, 1130–1132.
STEWART, K.K. 1980. Nutrient analysis of foods: State of the art for routine analyses, pp. 1–5. In Proceedings, Symposium on State of the Art for Routine Analyses. K.K. Stewart (Editor). Assoc. Official Anal. Chemists, Washington, D.C.
STOKES, J.L., Gunness, M., Dwyer, I.M., and Caswell, M.G. 1945. Microbiological methods for the determination of amino acids. J. Biol. Chem. 160, 35–49.
STOKSTAD, E.L.R., and Koch, J. 1967. Folic acid metabolism. Physiol. Rev. 47, 83–116.
STOKSTAD, E.L.R., and Thenen, S.W. 1972. Chemical and biochemical reactions of folic acid, pp. 387–408. In Analytical Microbiology, II. F. Kavanaugh (Editor). Academic Press, New York.
STOKSTAD, E.L.R., Shin, Y.S., and Tamura, T. 1977. Distribution of folate forms in food and folate availability, pp. 56–68. In Folic Acid: Biochemistry and Physiology in Relation to the Human Nutrition Requirement. Natl. Acad. Sci., Washington, D.C.
STOTT, J.A., and Smith, H. 1966. Microbiological assay of protein quality with Tetrahymena pyriformis W. Brit. J. Nutr. 20, 663–673.
Stott, J.A., Smith, H., and Rosen, G.D. 1963. Microbiological evaluation of protein quality with Tetrahymena pyriformis W. 3. A simplified assay procedure. Brit. J. Nutr. 17, 227–233.
TAMURA, T., Shin, Y.S., Williams, M.A., and Stokstad, E.L.R. 1972A. Lactobacillus casei response to pteroylpolyglutamates. Anal. Biochem. 49, 517–521.
TAMURA, T., Buehring, K.U., and Stokstad, E.L.R. 1972B. Enzymatic hydrolysis of pteroylpolyglutamates in cabbage. Proc. Soc. Exp. Biol. Med. 141, 1022–1025.
TAYLOR, S.L. 1982. Mutagenesis vs carcinogenesis. Food Technol. 36(3), 65–68, 98, 100, 102-103, 127.
TENNANT, G.B., and Withey, J.L. 1972. An assessment of work simplified procedures for the microbiological assay of serum vitamin B12 and serum folate. Med. Lab. Technol. 29, 171–181.
TEUNISSON, D.J. 1961. Microbiological assay of intact proteins using Tetrahymena pyriformis W. I. Survey of protein concentrates. Anal. Biochem. 2, 405–420.
TIGNER, J., and Roe, D.A. 1979. Tissue folacin stores in rats measured by radioassay. Proc. Soc. Exp. Biol. Med. 160, 445–448.
TOEPFER, E.W., and Polansky, M.M. 1970. Microbiological assay of vitamin B6 and its components. J. Assoc. Off. Anal. Chem. 53, 546–550.
TSUJI, K. 1966A. Liquid nitrogen preservation of Saccharomyces carlsbergensis and its use in a rapid biological assay of vitamin B6 (pyridoxine). Appl. Microbiol. 14, 456–461.
TSUJI, K. 1966B. Liquid nitrogen preservation of Saccharomyces carlsbergensis and its use in a rapid biological assay of pantothenic acid. Appl. Microbiol. 14, 462–465.
TSUJI, K., Elfring, G.L., Crain, H.H., and Cole, R.J. 1967. Dose response curve linearization and computer potency calculation of turbidimetric microbiological vitamin assays. Appl. Microbiol. 15, 363–367.
UNITED STATES PHARMACOPEIA, XIX. 1975. U.S.P. Conv., Inc., Rockville, Md. Voigt, M.S., and Eitenmiller, R.R. 1979. A liquid scintillation technique for microbial vitamin analyses. J. Food Sci. 44, 1780-1781.
VOIGT, M.N., Ware, G.O., and Eitenmiller, R.R. 1979A. Computer programs for the evaluation of vitamin B data obtained by microbiological methods. J. Agric. Food. Chem. 27, 1305–1311.
VOIGT, M.N., Eitenmiller, R.R., and Ware, G.O. 1979B. Vitamin analysis by microbial and protozoan organisms: Response to food preservatives and neutralization salts. J. Food Sci. 44, 723–737.
WADDELL, C.C., Domstad, P.A., Pircher, F.J., Lerner, S.R., Brown, J.A., and Lawhorn, B.K. 1976. Serum folate levels. Comparison of microbiologie assay and radioisotope kit methods. A.J. Clin.Path. 66, 746–752.
WANG, Y.Y.D., Miller, J., and Beuchat, L.R. 1979A. Comparison of Tetrahymena pyriformis W, Aspergillus flavus, and rat bioassays for evaluating protein quality of selected commercially prepared food products. J. Food Sci. 44, 1390–1393.
WANG, Y.Y.D., Miller, J., and Beuchat, L.R. 1979B. Comparison of four techniques for measuring growth of Tetrahymena pyriformis W with rat PER bioassays in assessing protein quality. J. Food Sci. 44, 540–544.
WARREN, R.M., and Labuza, T.P. 1977. Comparison of chemically measured available lysine with relative nutritive value measured by a Tetrahymena bioassay during early stages of nonenzymatic browning. J. Food Sci. 42, 429–431.
WAXMAN, S., Schreiber, C., and Herbert V. 1971. Radioisotopic assay for measurement of serum folate levels. Blood 38, 219–228.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 The AVI Publishing Company, Inc.
About this chapter
Cite this chapter
Jay, J.M. (1984). Microbiological Assays. In: Stewart, K.K., Whitaker, J.R. (eds) Modern Methods of Food Analysis. ift Basic Symposium Series. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-7379-7_10
Download citation
DOI: https://doi.org/10.1007/978-94-011-7379-7_10
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-011-7381-0
Online ISBN: 978-94-011-7379-7
eBook Packages: Springer Book Archive