Skip to main content
SpringerLink
Log in

Ultrastructural Aspects of the Intracellular Assembly, Transport and Exocytosis of Chylomicrons by Rat Intestinal Absorptive Cells

  • Chapter
Lipid Absorption: Biochemical and Clinical Aspects

Abstract

The mechanism by which the small intestinal epithelial cells absorb the products of intraluminal dietary fat digestion and transform them into secretory particles (chylomicrons) has intrigued physiologists and morphologists for over 100 years. Although the general features of lipid transport into the intracellular compartment of the small intestinal absorptive cells and the subsequent appearance of chylomicrons in the lymph were evident from light microscopic studies it required the development of electron microscopy and its utilization in correlative biochemical studies to elucidate the intimate details of the absorptive process.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Abbreviations

SER:

smooth endoplasmic reticulum

RER:

rough endoplasmic reticulum

LP:

lamina propria

IS:

intercellular space

G:

Golgi complex

MV:

microvilli

V:

vesicles

C:

chylomicrons

CIS:

cisternae

Ly:

lymphatic

References

  • Bauer, C. H., Lukaschek, R. and Reutter, W. G. (1974). Studies on the Golgi apparatus: Cumulative inhibition of protein and glycoprotein secretion by D-galactosamine. Biochem. J., 142, 221

    PubMed  CAS  Google Scholar 

  • Bergeron, J. J. M., Ehrenreich, J. H., Siekevitz, P. and Palade, G. E. (1973). Golgi functions prepared from rat liver homogenates. II. Biochemical characterization. J. Cell Biol., 59, 73

    Article  PubMed  CAS  Google Scholar 

  • Blomhoff, J. P. (1973). Serum cholesterol determination by gas-liquid chromatography. Clin. Chim. Acta, 43, 257

    Article  PubMed  CAS  Google Scholar 

  • Cardell, R. R., Jr., Badenhausen, S. and Porter, K. (1973). Intestinal triglyceride absorption in the rat. J. Cell. Biol., 34, 123

    Article  Google Scholar 

  • Casley-Smith, J. R. (1962). The identification of chylomicra and lipoproteins in tissue sections and their passage into jejunal lacteals. J. Cell Biol., 15, 259

    Article  PubMed  CAS  Google Scholar 

  • Clark, S. B., Lawergren, B. and Martin, J. V. (1973). Regional intestinal absorptive capacities for triolein: an alternative to markers. Amer. J. Physiol., 225, 574

    PubMed  CAS  Google Scholar 

  • Decker, K. and Keppler, D. (1973). Galactosamine-induced liver injury. In Popper and Schaffner (eds.). Progress in Liver Disease, Vol. IV, pp. 183–199. (New York: Grune and Stratton)

    Google Scholar 

  • Dobbins, W. O. (1966). An ultrastructural study of the intestinal mucosa in congenital β-lipoprotein deficiency with particular emphasis upon the intestinal absorptive cell. Gastroenterology, 50, 195

    Google Scholar 

  • Friedman, H. I. and Cardell, R. R., Jr. (1972). Effects of puromycin on the structure of rat intestinal epithelial cells during fat absorption. J. Cell Biol., 52, 15

    Article  PubMed  CAS  Google Scholar 

  • Glickman, R. M., Kirsch, K. and Isselbacher, K. J. (1972). Fat absorption during inhibition of protein synthesis: studies of lymph chylomicrons. J. Clin. Invest., 51, 356

    Article  PubMed  CAS  Google Scholar 

  • Gotto, A. M., Levy, R. I., John, K. and Fredrickson, D. S. (1971). On the protein defect in abetalipoproteinemia. N. Eng. J. Med., 284, 813

    Article  CAS  Google Scholar 

  • Isselbacher, K. J., Scheig, R., Plotkin, G. R. and Caulfield, J. B. (1964). Congenital β-lipoprotein deficiency: an hereditary disorder involving a defect in the absorption and transport of lipids. Medicine, 43, 347

    PubMed  CAS  Google Scholar 

  • Jamieson, J. D. and Palade, G. E. (1967). Intracellular transport of secretory proteins in the pancreatic exocrine cell. I. Role of the peripheral elements of the Golgi complex. J. Cell Biol., 34, 577

    Article  PubMed  CAS  Google Scholar 

  • Katterman, R. and Wolfrum, D. J. (1970). Cholesterinstoffwechsel und Lecithin-Cholesterin-Acyltransferase in Plasma bei Experimenteller Hepatitis und Cholestase an der Ratte. Z. Klin. Chem., 8, 413

    Google Scholar 

  • Keppler, D., Lesch, R., Reutter, W. and Decker, K. (1968). Experimental hepatitis induced by D-galactosamine. Exp. Mol. Pathol., 9, 279

    Article  PubMed  CAS  Google Scholar 

  • Kessler, J. I., Narcessian, P. and Mauldin, D. P. (1975). Biosynthesis of lipoproteins by intestinal epithelium. Site of synthesis and sequence of association of lipid, sugar and protein moieties. Gastroenterology, 68, 1058

    Google Scholar 

  • Koff, R. S., Gordon, G. and Sabesin, S. M. (1971(a)) D-galactosamine hepatitis. I. Hepatocellular injury and fatty liver following a single dose. Proc. Soc. Exp. Biol Med., 137, 696

    CAS  Google Scholar 

  • Koff, R. S., Fitts, J. J. and Sabesin, S. M. (1971(b)) D-galactosamine hepatotoxicity. II. Mechanism of fatty liver production. Proc. Soc. Exp. Biol. Med., 138, 89

    PubMed  CAS  Google Scholar 

  • Kostner, G., Holasek, A., Bohlmann, H. G. and Thiede, H. (1974). Investigation of serum lipoproteins and apoproteins in abetalipoproteinemia. Clin. Sci. Mol. Med., 46, 457

    PubMed  CAS  Google Scholar 

  • Lacy, P. E., Howell, S. L. Young, D. A., and Fink, C. J. (1968). New hypothesis of insulin secretion. Nature (London), 219, 1177

    Article  CAS  Google Scholar 

  • LeMarchand, Y., Singh, A., Assimacopoulos-Jeannet, F., Orci, L., Rouiller, C. and Jeanrenaud, B. (1973). A role for the microtubular system in the release of very low density lipoproteins by perfused mouse livers. J. Biol. Chem., 248, 6862

    CAS  Google Scholar 

  • Lo, C-H. and Marsh, J. B. (1970). Incorporation of [14C]glucosamine by cells and subcellular fractions of rat liver. J. Biol. Chem., 245, 5001

    PubMed  CAS  Google Scholar 

  • Redgrave, T. G. (1971). Association of Golgi membranes with lipid droplets (pre-chylomicrons) from within intestinal epithelial cells during absorption of fat. Aust. J. Exp. Biol. Med. Sci., 49, 209

    Article  PubMed  CAS  Google Scholar 

  • Redgrave, T. G. and Zilversmit, D. B. (1969). Does puromycin block release of chylomicrons from the intestine? Amer. J. Physiol., 217, 336

    PubMed  CAS  Google Scholar 

  • Sabesin, S. M. and Isselbacher, K. J. (1965). Protein synthesis inhibition: mechanism for production of impaired fat absorption. Science, 147, 1149

    Article  PubMed  CAS  Google Scholar 

  • Sabesin, S. M., Holt, P. R. and Clark, S. B. (1975(a)). Intestinal lipid absorption: Evidence for an intrinsic defect in chylomicron secretion in normal rat distal intestine. Lipids, 10, 840

    Article  PubMed  CAS  Google Scholar 

  • Sabesin, S. M., Kuiken, L. and Ragland, J. B. (1975(b)). Lipoproteins and lecithin: cholesterol acyltransferase changes in galactosamine induced rat liver injury. Science, 190, 1302

    Google Scholar 

  • Saloni, F. G. (1971). Simplified manual micromethod for determination of serum triglycerides. Clin. Chem., 17, 529

    Google Scholar 

  • Schachter, H., Jabbal, I., Hudgin, R. L. and Pinteric, L. (1970). Intracellular localization of liver sugar nucleotide glycoprotein glycosyltransferases in a Golgi-rich fraction. J. Biol. Chem., 245, 1090

    PubMed  CAS  Google Scholar 

  • Schachter, H. and Rodén, L. (1973). The biosynthesis of animal glycoproteins. In Fishman (ed.). Metabolic Conjugation and Metabolic Hydrolysis, Vol. III, pp. 2–149. (New York: Academic Press)

    Google Scholar 

  • Shinozuka, H., Farber, J. L., Konishi, Y. and Anukarahanonta, T. (1973). D-galactosamine and acute liver injury. Fed. Proc., 32, 1516

    PubMed  CAS  Google Scholar 

  • Simmonds, W. J. (1972). Fat absorption and chylomicron formation. In: Nelson (ed.). Lipids and Lipoproteins, pp. 705–743. (New York: Wiley)

    Google Scholar 

  • Stein, O., Sanger, L. and Stein, Y. (1974). Colchicine-induced inhibition of lipoprotein and protein secretion into the serum and lack of interference with secretion of biliary phospholipids and cholesterol by rat liver in vivo. J. Cell Biol., 62, 90

    Article  PubMed  CAS  Google Scholar 

  • Stokke, K. T. and Norum, K. R. (1971). Determination of lecithin: cholesterol acyltransferase in human blood plasma. Scand. J. Clin. Lab. Invest., 27, 21

    Article  PubMed  CAS  Google Scholar 

  • Strauss, E. W. (1966(a)). Electron microscopic study of intestinal fat absorption in vitro, from mixed micelles containing linoleic acid, monolein and bile salt. J. Lipid Res., 7, 307

    PubMed  CAS  Google Scholar 

  • Strauss, E. W. (1966(b)). Morphological aspects of triglyceride absorption. In: Charles F. Code (ed.). Handbook of Physiology, Vol. 3, pp. 1377–1406. (Washington, D.C.: Amer. Physiol. Soc.)

    Google Scholar 

  • Yousef, I. M. and Kuksis, A. (1972). Release of chylomicrons by isolated cells of rat intestinal mucosa. Lipids, 7, 380

    Article  PubMed  CAS  Google Scholar 

  • Zak, B. (1957). Simple rapid microtechnic for serum total cholesterol. Amer. J. Clin. Path., 27, 583

    CAS  Google Scholar 

Download references

Authors
  1. S. M. Sabesin
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Ulm, Germany

    K. Rommel  & R. Böhmer  & 

  2. University of Essen, Germany

    H. Goebell

Rights and permissions

Reprints and permissions

Copyright information

© 1976 MTP Press Ltd

About this chapter

Cite this chapter

Sabesin, S.M. (1976). Ultrastructural Aspects of the Intracellular Assembly, Transport and Exocytosis of Chylomicrons by Rat Intestinal Absorptive Cells. In: Rommel, K., Goebell, H., Böhmer, R. (eds) Lipid Absorption: Biochemical and Clinical Aspects. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-7176-2_9

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-7176-2_9

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-011-7178-6

  • Online ISBN: 978-94-011-7176-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation