Abstract
Root morphology is both genetically programmed and environmentally determined. We have begun an analysis into the components of root development by: (a) constructing a range of transgenic clover plants to assess some of the genetic programs involved as both roots and nodules are initiated and develop. These transgenic plants report on auxin activity, flavonoid synthesis and chitinase expression and suggest a role for flavonoids as regulators of auxin levels; and (b) determining in cereals the effect of both added auxin and specific microorganisms on the initiation and development of modified root outgrowths and lateral roots. Appropriate combinations of auxin, the nitrogen fixing Acetobacter diazotrophicus, and rice variety did give rise to some plants which grew slowly for over 12 months in a nitrogen-free medium.
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Abbreviations
- CHS:
-
chalcone synthase, 2, 4-D — 2,4-Dichlorophenoxyacetic acid
- GUS:
-
β-glucuronidase
- MRO:
-
modified root outgrowth
- NFM:
-
nitrogen free medium
- RCA:
-
rooted cotyledon assay
- RLA:
-
rooted leaf assay
- X-giuc:
-
5-bromo-4-chloro-3-indolyl β-D-glucuronic acid
References
Arioli T, Howles P A Weinman J J and Rolfe B G 1994 In Trifolium subterraneum, chalcone synthase is encoded by a multigene family. Gene 138, 79–86.
Ashford A E and McCully M E 1970 Locatization of Naphthol AS-B1 phosphatase activity in lateral and main root meristems of pea and corn. Protoplasma 70, 441–456.
Bell J K and McCully M E 1970 A Histological Study of lateral root initiation and development in Zea mays. Protoplasma 70, 179–205.
Bender G L 1988 PhD Thesis, The genetic basis of Rhizobium host range extension to the non-legume Parasponia. Australian National University.
Benfey P N and Schiefelbein J W 1994 Getting to the root of plant development: the genetics of Arabidopsis root formation. TIG 10, 84–88.
Cavalcante V A and Dobereiner J 1988 A new acid-tolerant nitrogen fixing bacterium associated with sugarcane. Plant Soil 108,23— 31.
Chasan R 1993 Embryogenesis: new molecular insights. The Plant Cell 5, 597–599.
Dobereiner J, Reis V M, Paula M A and Olivares F 1993 Endophytic diazotrophs in sugar cane, cereals and tuber plants. In New Horizons in Nitrogen Fixation. Eds. R Palacios, Mora, W Newton. pp. 671–676. Kluwer Academic Publishers, Dordrecht, The Netherlands.
Dolan L, Janmaat K, Willemsen V, Linstead P, Poethig S, Roberts K and Scheres B 1993 Cellular organisation of the Arabidopsis thaliana root. Development 119, 71–84.
Dong Z, Canny M J, McCully M E, Roboredo M R, Cabadilla C F, Ortega E and Rodes R 1994 A nitrogen-fixing endophyte of sugarcane stems. Plant Physiol. 105, 1139–1147.
Dreyfus B, Garcia J L and Gillis M 1988 Characterization of Azorhizobium caulinodans. Gen. Nov. sp. Nov, a stem-nodulating nitrogen-fixing bacterium isolated from Sesbania rostrata. Int. Systemic Bacteriol. 38, 89–98.
Duditz D, Börge L, Bakó L, Dedeoglu D, Magyar Z, Kapros T, Felföldi F and Györgyey J 1993 Key components in cell cycle control during auxin-induced cell division. In: Molecular and cell biology of the cell cycle. Eds. J C Ormrod and D Francis. pp Ill-131. Kluwer Academic Publishers, Dordrecht, The Netherlands.
Fitter A H 1991 Characteristics and functions of root systems. In Plant Roots: The Hidden Half. Eds. Y Waisel, A Eshel and U Kafkafi. pp. 3–25. Marcel Dekker, Inc., New York.
Gallagher S R 1992 GUS protocols: Using the GUS gene as a reporter of gene expression. Academic Press.
Gochnauer M B, McCully M E and Labbe H 1989 Different populations of bacteria associated with sheathed and bare regions of roots of field-grown maize. Plant Soil 114, 107–120.
Hagen G, Martin G, Li Y and Guilfoyle T J 1991 Auxin-induced expression of the soybean GH3 promoter in transgenic tobacco plants. Plant Molec. Biol. 17, 567–569.
Hirsch A M 1992 Developmental biology of legume nodulation. New Phytol. 122, 211–237.
Bowles P A, Arioli T and Weinman J J 1994 Characterization of a phenylalanine ammonia-lyase multigene family in Trifolium subteraneum. Gene 138, 87–92.
Jacobs M and Rubery P H 1988 Naturally occurring auxin transport regulators. Science 241, 346–349.
Jefferson R A, Kavanagh T A and Bevan M W 1987 GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6, 3901–3907.
Key J L 1989 Modulation of gene expression by auxin. BioEssays 11, 52–57.
Khan M R I, Tabe L M, Heath L C, Spencer D and Higgins T J V 1994 Agrobacterium-mediated transformation of subterranean clover (Trifolium subterraneum L.). Plant Physiol. 105, 81–88.
Larkin P J, Gibson J M, Mathesius U, Weinman J J, Gärtner E, Hall E, Tanner G J, Rolfe B G and Djordjevic M A 1996 Transgenic white clover. Studies with the auxin responsive promoter, GH3, in root gravitropism and secondary root development. Transgenic Research 5, 325–335.
Lawson C G R, Djordjevic M A, Weinman J J and Rolfe B G 1994 Rhizobium inoculation and physical wounding results in the rapid induction of the same chalcone synthase copy in Trifolium subterraneum. Molec. Plant-Microbe Interact. 7, 498–507.
le Strange K K, Bender G L, Djordjevic M A, Rolfe B G and Redmond J W 1990 The Rhizobium strain NGR234 nodDl gene product responds to activation by the simple phenolic compounds vanillin and isovanillin present in wheat seedling extracts. Molec. Plant-Microbe Interact. 3, 214–220.
Lie T A 1971 Nodulation of rooted leaves in leguminous plants. Plant and Soil 34, 663–673.
Mathesius U, Schlaman H R M, Meijer D, Lugtenberg B J J, Spaink H P, Weinman J J, Roddam L F, Sautter C, Rolfe B G and Djordjevic M A 1996 New tools for investigating nodule initiation and ontogeny: Spot inoculation and microtargeting of transgenic white clover roots shows auxin involvement and suggests a role for flavonoids. 8th International Congress Molecular Plant-Microbe Interactions, Knoxville, TN.
Neale A D, Wahleithner J A, Lund M, Bonnett H T, Kelly A, Meeks-Wagner D R, Peacock W J and Dennis E S 1990 Chitinase, ß-1,3-glucanase, osmotin, and extensin are expressed in tobacco explants during flower formation. The Plant Cell 2, 673–684.
Oka H 11988 Origin of cultivated rice, Japan Scientific Societies Press/Elsevier, Tokyo.
Ridge R W, Bender G L and Rolfe B G 1992 Nodule-like structures induced on the roots of wheat seedlings by the addition of the synthetic auxin 2,4-Dichlorophenoxyacetic acid and the effects of microorganisms. Aust. J. Plant Physiol, 19, 481–492.
Ridge R W, Ride K M and Rolfe B G 1993 Nodule-like structures induced on the roots of rice seedlings by addition of the synthetic auxin 2,4-Dichlorophenoxyacetic acid. Aust. J. Plant Physiol., 20, 705–717.
Rolfe B G and McIver J M 1994 The rooted cotyledon and rooted leaf bioassay. Proc. 1st European Nitrogen Fixation Conference, Szeged, Hungary, 122–126.
Rolfe B G and McIver J M 1996 Single-leaf plant bioassays for the study of root morphogenesis and Rhizobium-legume nodulation. Aust. J. Plant Physiol., 23, 271–283.
Sachs T 1993 The role of auxin in the polar organization of apical meristems. Aust. J. Plant Physiol. 20, 541–553.
Sachs T 1984 Positional Controls in Plant Development. Eds. P W Barlow and D J Carr. pp. 193–224. Cambridge University Press, Cambridge, UK.
Schiefelbein J W and Benfey P N 1991 The development of plant roots: New approaches to underground problems. The Plant Cell 3, 1147–1154.
Sussex I M, Godoy J A, Kerk N M, Laskowski M J, Nusbaum H C, Welsch J A and Williams M E 1995 Cellular and molecular events in a newly organizing lateral root meristem. Phil. Trans. R. Soc. Lond. B 350, 39–43.
Trinick M J 1980 Relations among the fast-growing rhizobia of
Lablab purpureus, Leucaena leucocephala, Mimosa spp., Acacia farnesiana and Sesbania grandiflora and their affinities with other rhizobial groups. J. Appl. Bacteriol. 49, 39–53.
Tsunoda S and Takahashi N 1984 Biology of rice, Japan Scientific Societies Press, Elsevier, Tokyo.
Warren-Wilson J and Warren-Wilson P M 1993 Mechanisms of auxin regulation of structural and physiologic polarity in plants, tissues, cells and embryos. Aust. J. Plant Physiol. 20, 555–571.
Whiteman F, Schneider E and Thimann K V 1980 Hormonal factors controlling the initiation and development of lateral roots. Physiol. Plant. 49, 304–314.
Wilson K J 1995 Molecular techniques for the study of rhizobial ecology in the field. Soil Biology and Biochemistry 27, 501–514.
Zobel R W 1991 Genetic control of root systems. In Plant Roots: The Hidden Half. Eds. Y Waisel, A Eshel and U Kafkafi. pp. 27–38. Marcel Dekker, Inc., New York.
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Rolfe, B.G. et al. (1997). Root morphogenesis in legumes and cereals and the effect of bacterial inoculation on root development. In: Ladha, J.K., de Bruijn, F.J., Malik, K.A. (eds) Opportunities for Biological Nitrogen Fixation in Rice and Other Non-Legumes. Developments in Plant and Soil Sciences, vol 75. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-7113-7_13
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DOI: https://doi.org/10.1007/978-94-011-7113-7_13
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