Abstract
We wish to develop reagents for the sequence-specific cleavage of RNA by non-oxidative mechanisms. The molecules we have devised may be described as synthetic ribozymes, and comprise a molecular recognition agent (such as single-stranded DNA) and an RNA hydrolysis agent. Their potential applications include antiviral or antifungal therapy, and they were designed as first-generation catalytic antisense DNA drugs for the control of gene expression. We provide a brief review of the principles of the antisense method, a discussion of the importance (for in vivo applications) of hydrolytic cleavage as an alternative to oxidative degradation of nucleic acids, and a discussion of our synthetic and mechanistic efforts toward synthetic ribozymes.
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Bashkin, J.K. (1993). RNA Hydrolysis by Cu(II) Complexes: Toward Synthetic Ribonucleases and Ribozymes. In: Karlin, K.D., Tyeklár, Z. (eds) Bioinorganic Chemistry of Copper. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-6875-5_11
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DOI: https://doi.org/10.1007/978-94-011-6875-5_11
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