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Analytical Electrophoretic Techniques in Protein Chemistry

  • Bryan John Smith
  • Robert H. Nicolas

Abstract

The method often chosen today for analysis of proteins is electrophoresis in polyacrylamide gels. Microgram amounts of material can be used to give information on a protein’s molecular weight, isoelectric point, etc. Many electrophoretic techniques have been described in the literature, but this chapter describes just some of the most commonly used of them. The general principles and practice of electrophoresis are also described but relate mainly to the methods discussed. For more comprehensive treatments of the subject, the reader is directed to reviews such as those by Smith1 and Gordon2 and references therein. Other references are given below for particular points not covered at length in these reviews.

Keywords

Electrophoretic Mobility Crosslin King Agent Electrophoretic Technique Ammonium Persulphate Acrylamide Content 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© John M. Walker and Wim Gaastra 1983

Authors and Affiliations

  • Bryan John Smith
  • Robert H. Nicolas

There are no affiliations available

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