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Detection of Specific DNA Sequences — The Southern Blot

  • Christopher G. P. Mathew

Abstract

In a previous chapter (Chapter 14) the characterisation of DNA by restriction analysis on agarose gels has been described. In such cases the DNA sample would normally be about one microgram of a single type of DNA sequence. However, if you wished to analyse the structure of a specific gene from a complex organism without prior purification of the gene, you would have to detect picogram amounts of a single type of DNA sequence among about a million other types. The method used would therefore have to be very sensitive and very specific. This exacting task can be accomplished by means of a technique devised by Edwin Southern of Edinburgh University.1 The technique has come to be known as the Southern transfer or Southern blot, and has had an enormous impact on molecular biology and genetics.

Keywords

Southern Blot Sickle Cell Anaemia Globin Gene Nitrocellulose Filter Antenatal Diagnosis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Further Reading

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    P. Chambon, ‘Split genes’, Sci. Amer., 244 (1981), 48–59CrossRefGoogle Scholar
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    B.D. Hall. L. Haar and K. Klepp, ‘Development of the nitrocellulose filter technique for RNA-DNA hybridisation’, Trends in Biochemical Sciences, 5 (1980), 254–256CrossRefGoogle Scholar
  3. 3.
    P.F.R. Little, ‘Ante-natel diagnosis of haemoglobinopathies’ in R. Williamson (ed.), Genetic Engineering, (Academic Press. 1981 ) vol. 1, 61-102.Google Scholar

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Copyright information

© John M. Walker and Wim Gaastra 1983

Authors and Affiliations

  • Christopher G. P. Mathew

There are no affiliations available

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