Abstract
Bacteria possess restriction-modification systems, each system of which consists of a modification methylase and a restriction endonuclease. The modification methylase recognises a specific sequence and methylates certain adenines or cytosines within this sequence. The corresponding restriction endonuclease recognises the same sequence and in most cases cleaves the DNA when the sequence has not been methylated by the modification methylase.
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Further Reading
Methods in Enzymology. ‘Nucleic Acids’. Eds: L. Grossman and K. Moldave (Academic Press. 1980). 65.
Methods in Enzymology. ‘Recombinant DNA’. Ed: R. Wu (Acadcmic press. 1979). 68.
A. Kornberg. ‘DNA Rcplication’ (Frceman and Co.).
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© 1983 John M. Walker and Wim Gaastra
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Mooi, F.R., Gaastra, W. (1983). The Use of Restriction Endonucleases and T4 DNA Ligase. In: Walker, J.M., Gaastra, W. (eds) Techniques in Molecular Biology. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-6563-1_11
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DOI: https://doi.org/10.1007/978-94-011-6563-1_11
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