Abstract
Bombardment-based methodology is responsible for the effective genetic manipulation of major cereals including rice. Many groups reported significant advances on various aspects of rice molecular biology and genetic engineering using procedures based on bombardment technology. Molecular and genetic characterization of large numbers of these plants (more than 500 independent transgenic plants) provided information on structure, expression and stability of integrated DNA through multiple generations. Such evaluations were carried out in the greenhouse and in the field. Stability of expression was found to be dependent on the nature of the promoter and the transgene, and in specific cases on gene copy number. Direct DNA transfer utilizing particle bombardment for the delivery of foreign DNA into rice tissue results in the recovery of large numbers of independently derived transgenic plants in a variety-independent fashion. Gene copy number, level and stability of expression of transgenes can be compared to other DNA delivery methods, direct or indirect, including Agrobacterium-mediated gene transfer. In this paper, the technology is summarized and discussed in terms of present and future applications, including field trials and potential commercialization of transgenic rice expressing a number of genes of agronomic interest such as pest and herbicide resistance.
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References
Anonymous: The Rockefeller Foundation solicits research proposals on the genetic engineering of rice. Plant Mol Biol Rep 3: 145–146 (1985).
Zhang W, Wu R: Efficient regeneration of transgenic plants from rice protoplasts and correctly regulated expression of the foreign gene in the plants. Theor Appl Genet 76: 835–840 (1988).
Toriyama K, Arimoto Y, Uchimiya H, Hinata K: Trans-genie rice plants after direct gene transfer into protoplasts. Bio/technology 6: 1072–1074 (1988).
Zhang HM, Yang H, Rech EL, Golds TJ, Davis AS, Mulligan BJ, Cocking EC: Transgenic rice plants produced by electroporation mediated plasmid uptake into protoplasts. Plant Cell Rep 7: 379–383 (1988).
Ou-Lee TM, Turgeon R, Wu R: Expression of a foreign gene linked to either a plant virus or a Drosophila promoter after electroporation of protoplasts of rice, wheat and sorghum. Proc Natl Acad Sci USA 83: 6815–6819 (1986).
Uchimiya H, Fushirni T, Hashimoto H, Harada H, Syono K, Sugawara Y: Expression of a foreign gene in callus derived from DNA-treated protoplasts of rice (Oryza sativa L.). Mol Gen Genet 16: 204–207 (1986).
Datta SK, Peterhans A, Datta K, Potrykus I: Genetically engineered fertile Indica-Rice recovered from protoplasts. Bio/technology 8: 736–740 (1990).
Christou P, Ford TL, Kofron M: Production of transgenic rice (Oryza sativa L.) plants from agronomically important indica and japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos. Bio/technology 9: 957–962 (1991).
Christou P, Ford TL, Kofron M: Rice genetic engineering: a review. Trends Biotechnol 10: 239–246 (1992).
Hiei Y, Ohta S, Komari T, Kumashiro T: Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. Plant J 6: 271–282 (1994).
Oard JH, Linscombe SD, Braverman MP, Jodari F, Bollich PK, Blouin DC, Cooley JC, Leech M, Kholi A, Vain P, Christou P: Development, field evaluation and agronomic performance of transgenic herbicide resistant rice. J Mol Breed, in press (1996).
Sanford JC: The biolistic process. Trends Biotechnol 6: 299–302 (1988).
Christou P, Ford T: Recovery of chimeric rice plants from dry seed using electric discharge particle acceleration. Ann Bot 75: 449–454 (1995).
Christou P, Ford TL: Parameters influencing stable transformation of rice embryonic tissue and recovery of transgenic plants using electric discharge particle acceleration. Ann Bot 75: 407–413 (1995).
Christou P, Ford TL, Kofron M: The impact of selection parameters on the phenotype and genotype of transgenic rice callus and plants. Transgen Res 4: 44–51 (1995).
Cao J, Duan X, McElroy D, Wu R: Regeneration of herbicide resistant transgenic rice plants following microprojectilemediated transformation of suspension culture cells. Plant Cell Rep 11: 586–591 (1992).
Li L, Qu R, de Kochko A, Fauquet C, Beachy RN: An improved rice transformation system using the biolistic method. Plant Cell Rep 12: 250–255 (1993).
Russell JA, Roy MK. Sanford JC: Physical trauma and tungsten toxicity reduce the efficiency of biolistic transformation. Plant Physiol 98: 1050–1056 (1992).
McCabe DE, Christou P: Direct DNA transfer using electric discharge particle acceleration (AccellR) technology. Plant Cell Tissue Organ Culture 33: 227–236 (1993).
Kalla R, Shimamoto K, Potter R, Nielsen PS, Linnestad C, Olsen OA: The promoter of the barley aleurone-specific gene encoding a putative 7 kDa lipid transfer protein confers aleurone cell-specific expression in transgenic rice. Plant J 6: 849–860 (1994).
Sivamani E, Shen P, Opalka N, Beachy RN, Fauquet CM: Selection of large quantities of embryogenic calli from indica rice seeds for production of fertile transgenic plants using the biolistic method. Plant Cell Rep 15: 322–327 (1996).
Zhang S, Chen L, Qu R, Marmey P, Beachy, Fauquet CM: egeneration of fertile transgenic indica (group 1) rice plants following microprojectile transformation of embryogenic suspension culture cells. Plant Cell Rep 15: 465–469 (1996).
Wunn J, Kloti A, Burkhardt PK, Ghosh Biswas GC, Launis K, Iglesias VA, Potrykus I: Transgenic indica rice breeding line IR58 expressing a synthetic crylA9b) gene from Bacillus thuringiensis provides effective insect pest control. Bio/technology 14: 171–176 (1996).
Cooley J, Ford TL, Christou P: Molecular and genetic charac-terization of elite transgenic rice plants produced by electric discharge particle bombardment. Theor Appl Genet 90: 97–104 (1995).
Christou P, Swain WF: Cotransformation frequencies of foreign genes in soybean cell cultures. Theor Appl Genet 79: 337–341 (1990).
Kartzke S, Saedler H, Meyer P: Molecular analysis of transgenic plants derived from transformants of protoplasts at various stages of the cell cycle. Plant Sci 67: 63–72 (1990).
Saul MW, Potrykus I: Direct gene transfer to protoplasts: fate of the transferred genes. Devel Genet 11: 176–181 (1990).
Ramanathan V, Veluthambi K: Transfer of non-T-DNA portions of Agrobacterium tumefaciens Ti plasmid pTiA6 from theh left terminus of TL-DNA. Plant Mol Biol 28: 1149–1154 (1995).
Matzik A, Mantell S, Schiemann J: Localization of persisting Agrobacteria in transgenic tobacco plants. Mol Plant-Microb Interact 9: 373–381 (1996).
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© 1997 Springer Science+Business Media Dordrecht
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Christou, P. (1997). Rice transformation: bombardment. In: Sasaki, T., Moore, G. (eds) Oryza: From Molecule to Plant. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5794-0_19
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DOI: https://doi.org/10.1007/978-94-011-5794-0_19
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