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QTL analysis to study the association between leaf size and abscisic acid accumulation in droughted rice leaves and comparisons across cereals

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Oryza: From Molecule to Plant

Abstract

Plants accumulate abscisic acid (ABA) under droughted conditions. Genetic variation in the accumulation of ABA in detached and partially dehydrated leaves of rice has previously been reported, and this was found to be associated with variation in leaf size (smaller leaves made more ABA). Correlation analysis failed to distinguish clearly between a causal relationship between the two traits and close genetic linkage between loci controlling the traits. Here we present a detailed genetic analysis of ABA accumulation in detached and partially dehydrated rice leaves, using a population of F2 plants generated from the lowland × upland cross IR20 (high-ABA) × 63–83 (low-ABA) which was mapped with RFLP and AFLP markers. Several highly significant quantitative trait loci (QTLs) for ABA accumulation and leaf weight were identified. Only one of the minor QTLs for ABA accumulation (accounting for only 4% of the phenotypic variance) was coincident with any QTLs for leaf size such that the high-ABA allele was associated with smaller leaves. This analysis, therefore, showed that the association previously found between ABA accumulation and leaf size was probably largely due to genetic linkage and not to a direct effect of leaf size on ABA accumulation or vice versa. Because of the importance of ABA accumulation in regulating responses of plants to drought stress and the effects of plant size on the rate of development of stress, QTLs for drought-induced ABA accumulation, leaf size and tiller number were compared between rice and wheat. In particular, a possible location in rice was sought for a homoeologue of the major wheat vernalization responsive gene, Vrnl, as this gene is also associated with major effects on leaf size, tiller number and ABA accumulation in wheat. The likelihood of homoeologous loci regulating ABA accumulation, leaf size and tiller number in the two crops is discussed.

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Authors and Affiliations

  1. John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK

    Stephen A. Quarrie, David A. Laurie, Jiahui Zhu, Claude Lebreton, Andrei Semikhodskii & Andrew Steed

  2. KeyGene n.v., Agro Business Park 90, P.O. Box 216, 6700 AE, Wageningen, Netherlands

    Hanneke Witsenboer

  3. Dipartimento delle Scienze Ambientale, Università degli Studi di Parma, Viale delle Scienze, 43100, Parma, Italy

    Christina Calestani

Authors
  1. Stephen A. Quarrie
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  2. David A. Laurie
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  3. Jiahui Zhu
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  4. Claude Lebreton
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  5. Andrei Semikhodskii
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  6. Andrew Steed
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  7. Hanneke Witsenboer
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  8. Christina Calestani
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Takuji Sasaki Graham Moore

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© 1997 Springer Science+Business Media Dordrecht

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Quarrie, S.A. et al. (1997). QTL analysis to study the association between leaf size and abscisic acid accumulation in droughted rice leaves and comparisons across cereals. In: Sasaki, T., Moore, G. (eds) Oryza: From Molecule to Plant. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5794-0_15

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  • DOI: https://doi.org/10.1007/978-94-011-5794-0_15

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6446-0

  • Online ISBN: 978-94-011-5794-0

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