Abstract
Late globular/early torpedo stage date palm embryoids can continue normal growth and development after cryopreservation provided they are pre—treated with a cryoprotectant mixture of glycerol and sucrose and then dried to water contents in the range of 0.4–0.7g.g-1. The embryoids were frozen by direct immersion in liquid nitrogen. Although further drying allows for 100% recovery, growth is in the form of unorganized callus. Tissues frozen at extremely rapid rates (by immersion in liquid freon) retain cytoskeletal structures, whereas material frozen at slower rates (in liquid nitrogen) appear to lose this subcellular system. The slow recovery rate of material frozen in liquid nitrogen could, in part, be due to reconstitution of this subcellular matrix.
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Mycock, D.J., Berjak, P., Pammenter, N.W., Vertucci, C.W. (1997). Cryopreservation of Somatic Embryoids of Phoenix dactylifera . In: Ellis, R.H., Black, M., Murdoch, A.J., Hong, T.D. (eds) Basic and Applied Aspects of Seed Biology. Current Plant Science and Biotechnology in Agriculture, vol 30. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5716-2_8
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DOI: https://doi.org/10.1007/978-94-011-5716-2_8
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