Abstract
During development of peanut seeds, a set of low molecular weight polypeptides was identified most of which were heat-stable. For wampee seeds, only one band of heat-stable proteins could be checked out by SDS-PAGE. It had a molecular weight of 20 kDa and constituted 1% of the total proteins. By Western blot analysis, wampee seed axes can express dehydrin-like proteins (at 67–88 days (d) after anthesis) but the level was very low. Stachyose and sucrose increased and maltose declined with the acquisition of desiccation tolerance during embryogenesis of peanut seed. Following the development of wampee seed, glucose and maltose decreased gradually, while fructose, sucrose and stachyose increased markedly. It may be assumed that the heat-stable and low molecular weight polypeptides synergize with soluble sugars in the acquisition of desiccation tolerance in peanut seed though not in wampee seed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Blackman, S.A.,Wettlaufer, S.H., Obendorf, R.L. and Leopold, A.C. 1991. Plant Physiology 96: 868–874.
Blackman, S.A., Obendorf, R.L. and Leopold, A.C. 1992. Plant Physiology 100: 225–230.
Bradford, M.M. 1976. Analytical Biochemistry 72: 248–252.
Caffrey, M., Fonseca, V. and Leopold, A.C. 1988. Plant Physiology 86: 754–758.
Crowe, J.H., Crowe, L.M. and Chapman, D. 1984. Science 223: 701–703.
Crowe, J.H., Crowe, L.M., Carpenter, J.F., Rudolph, A.S., Aurell Wistrom, C, Spargo, B.J. and Anchordoguy, T.J. 1988. Biochimica et Biophysica Acta 947: 367–384.
Crowe, JH., Hoekstra, F.A. and Crowe, L.M. 1992. Annual Review of Physiology 54: 579–599.
Farrant, J.M., Berjak, P. and Pammenter, N.W. 1992.Plant Growth Regulation 11: 257–265.
Farrant, J.M., Pammenter, N.W. and Berjak, P. 1993. Seed Science Research 3: 1–13.
Finch-Savage, W.E. 1992. Seed Science Research 2:17–22.
Finch-Savage, W.E. 1994. Abstracts of International Workshop on Desiccation Tolerance and Sensitivity of Seeds and Vegetative Plant Tissues, pp. 20, South Africa.
Fu, J.R., Zhang, B.Z., Wang, X.F. and Qiao, Y.Z. 1989a. In; 22nd International Seed Testing Congress, Abstracts, p.9, Zurich, ISTA Secretariat.
Fu, J.R., Zhang, B.Z., Wang, X.F., Qiao, Y.Z. and Huang, X.L. 1989b. In: International Symposium of Horticultural Germplasm, Cultivated, Wild, Part I. Fruit Trees, pp. 121–125. Beijing: International Academic Publishers.
Fu, J.R., Jin, LP., Peng, Y.F. and Xia, Q.H. 1994. Seed Science Research 4: 257–261.
Gee, O.H., Probert, R.J. and Coomber, S.A. 1994. Seed Science Research 4: 135–141.
Hofmann, P. and Steiner, AM. 1989. Landwirtschaftliche Forschung 42: 310–323.
Koster, K.L. and Leopold, A.C. 1988. Plant Physiology 88: 829–832.
Laemmli, U.K. 1970. Nature 227: 680–685.
Le Page-Degivry, M.T. and Garello, G. 1991. Seed Science Research 1: 221–227.
Leprince, O., Bronchart, R. and Deltour, R. 1990. Plant Cell Environment 13: 539–546.
McCown, B.H. and Lloyd, G. 1981. Horticulture 16: 453–459
Roberts, E.H. 1973. Seed Science and Technology 1: 499–514.
Sambrook, J., Fritsch, E.F. and Manniatis, T. 1989. A Laboratory Manual, Cold Spring Harbor Laboratory Press.
Sun, W.Q. and Leopold, A.C. 1993. Physiologia Plantarum 87: 403–409.
Yamada, T., Aibara, S. and Morita, Y. 1979. Agricultural Biological Chemistry 43: 2563–2568.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Fu, J.R., Yang, X.Q., Jiang, X.C., He, J.X., Song, S.Q. (1997). Heat Stable Proteins and Desiccation Tolerance in Recalcitrant and Orthodox Seeds. In: Ellis, R.H., Black, M., Murdoch, A.J., Hong, T.D. (eds) Basic and Applied Aspects of Seed Biology. Current Plant Science and Biotechnology in Agriculture, vol 30. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5716-2_77
Download citation
DOI: https://doi.org/10.1007/978-94-011-5716-2_77
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6410-1
Online ISBN: 978-94-011-5716-2
eBook Packages: Springer Book Archive