Abstract
The influence of methyl jasmonate (JA-Me), a new plant growth regulator, and abscisic acid (ABA) on growth of callus and somatic embryogenesis in petiole-derived tissue cultures of Medicago sativa has been investigated.
A two-step protocol based on SH medium for somatic embryogenesis was used in the experiments. JA-Me and ABA (5, 50, 450 µM) were added to induction medium before autoclaving. The weight of plant tissue (petioles with callus) after 10 days of incubation on induction medium, prior to transfer to differentiation medium, was determined. After 7 weeks on differentiation medium, cultures were weighed and somatic embryos were counted.
Both JA-Me and ABA are considered to be inhibitors of the induction of callus growth and somatic embryogenesis in Medicago sativa cultures. An exposure to those inhibitors during induction stage reduced the number of somatic embryos obtained. JA-Me showed to be less active in the inhibition of callus growth and somatic embryogenesis than ABA.
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© 1997 Springer Science+Business Media Dordrecht
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Kepczynski, J., Florek, I. (1997). The Influence of JA-Me and ABA on Induction of Somatic Embryogenesis in Medicago sativa L. Tissue Cultures. In: Ellis, R.H., Black, M., Murdoch, A.J., Hong, T.D. (eds) Basic and Applied Aspects of Seed Biology. Current Plant Science and Biotechnology in Agriculture, vol 30. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5716-2_16
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DOI: https://doi.org/10.1007/978-94-011-5716-2_16
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