Abstract
The vlue of vibrational spectroscopy in the study of biopolymers has been greatly enhanced by adding the new dimension of optical activity [1]. The Raman optical activity (ROA) approach, which measures a small difference in the intensity of Raman scattering in right and left circularly polarized incident light, has been transformed by recent technical advances into a powerful new biochemical spectroscopy [2]. Since protein ROA spectra contains bands from loops and turns in addition to secondary structure, they contain information about the tertiary backbone fold and hence the three-dimensional solution structure. This, plus a special sensitivity to conformational mobility and the short timescale of the Raman scattering process, makes ROA ideal for studying dynamic aspects of structure in both non-native [3, 4] and native [5] states.
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© 1997 Springer Science+Business Media Dordrecht
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Barron, L.D., Wilson, G., Hecht, L., Blanch, E.W. (1997). A New Perspective on Protein Structure and Dynamics from Raman Optical Activity. In: Carmona, P., Navarro, R., Hernanz, A. (eds) Spectroscopy of Biological Molecules: Modern Trends. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5622-6_3
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DOI: https://doi.org/10.1007/978-94-011-5622-6_3
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