Abstract
A polyclonal antibody toward the borate-rhamnogalacturonan-II (RG-II) complex was raised in rabbits. The antibody recognizes RG-II exclusively in cell walls. Immunocytochemical studies reveal that the epitope is ubiquitous in cell walls of all the cells in radish (Rhaphanus sativus) roots. The label was denser proximal to the plasma membrane and was not detected in the middle lamella. These results suggests that the borate bridge between RG-II chains is important for the network formation of newly produced pectic polysaccharides, and that the bridge may not be involved in cell to cell adhesion.
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© 1997 Springer Science+Business Media Dordrecht
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Takasaki, M., Kawaguchi, S., Kobayashi, M., Takabe, K., Matoh, T. (1997). Immunocytochemistry of the borate-rhamnogalacturonan II complex in cell walls of radish roots. In: Bell, R.W., Rerkasem, B. (eds) Boron in Soils and Plants. Developments in Plant and Soil Sciences, vol 76. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5564-9_46
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DOI: https://doi.org/10.1007/978-94-011-5564-9_46
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6344-9
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