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In vitro reconstitution of the boron-polysaccharide complex purified from cultured tobacco BY-2 cells

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Boron in Soils and Plants

Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 76))

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Abstract

A boron-polysaccharide complex isolated from cell walls of cultured tobacco (Nicotiana tabacum) BY-2 is a dimeric rhamnogalacturonan II (RG-II) cross-linked by borate-diol esters, the same as in the radish (Raphanus sativus) root cell wall. This complex can be reconstituted spontaneously in vitro simply by mixing monomeric RG-II and boric acid at pH 4. Germanic acid substitutes for boric acid in the reconstitution to some extent.

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© 1997 Springer Science+Business Media Dordrecht

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Kobayashi, M., Matoh, T. (1997). In vitro reconstitution of the boron-polysaccharide complex purified from cultured tobacco BY-2 cells. In: Bell, R.W., Rerkasem, B. (eds) Boron in Soils and Plants. Developments in Plant and Soil Sciences, vol 76. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5564-9_45

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  • DOI: https://doi.org/10.1007/978-94-011-5564-9_45

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6344-9

  • Online ISBN: 978-94-011-5564-9

  • eBook Packages: Springer Book Archive

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