Abstract
The paper presents the data suggesting that boron (B) content in the cultured tobacco (Nicotiana tabacum) cells change during the stage of cell cycle. Cultured cell line, tobacco BY-2, was synchronized by treatments with aphidicolin and propyzamide. When the cells started to grow, cells were periodically collected at each stage of the cell cycle. To measure the B content, cells were dried and prepared as tablets and prompt gamma-ray analysis (PGA) with a cold neutron beam was performed. Flow cytometry (FCM) was carried out on the same cell fraction to analyze the cell cycle in more detail. There was a clear difference in B content with respect to the cell cycle stage. Boron content in the cells at G2+M phase was found to be about half of that at G0/G1.
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© 1997 Springer Science+Business Media Dordrecht
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Iikura, H., Kataoka, T., Tamada, M., Nakanishi, T.M., Yonezawa, C. (1997). Boron analysis at different stages of the cell cycle in cultured tobacco cells. In: Bell, R.W., Rerkasem, B. (eds) Boron in Soils and Plants. Developments in Plant and Soil Sciences, vol 76. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5564-9_12
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DOI: https://doi.org/10.1007/978-94-011-5564-9_12
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6344-9
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