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Chromosomal DNA Loops and Domain Organization of the Eukaryotic Genome

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Genome Structure and Function

Part of the book series: NATO ASI Series ((ASHT,volume 31))

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Abstract

Over twenty years ago it was found that chromosomal DNA in eukaryotic cell nuclei is organized into large loops by periodic attachment to the high salt-insoluble proteinous nuclear (chromosomal) matrix. The specificity of genomic DNA organization into loops has been intensively studied with the aim to find out whether they may constitute quasi-independent structural-functional units of the genome. These studies have resulted in conflicting findings and consequently in conflicting conclusions. In this paper different experimental approaches used to analyse the above problem will be critically reviewed. The discussion will be followed by the presentation of a novel approach for mapping DNA loop anchorage sites which has been developed in our laboratory. Based on the excision of whole DNA loops by topoisomerase II - mediated DNA cleavage at matrix attachment sites this approach seems to constitute a unique tool for analyzing the topological organization of chromosomal DNA in living cells.

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  1. Institute of Gene Biology, Laboratory of Structural and Functional Organization of Chromosomes, Vavilov St. 34/5, Moscow, 117334, Russia

    S. V. Razin

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  1. Institute of Biophysics, University of Genoa, Genoa, Italy

    Claudio Nicolini

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Razin, S.V. (1997). Chromosomal DNA Loops and Domain Organization of the Eukaryotic Genome. In: Nicolini, C. (eds) Genome Structure and Function. NATO ASI Series, vol 31. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5550-2_2

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  • DOI: https://doi.org/10.1007/978-94-011-5550-2_2

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