Abstract
The work presented in the oral paper comprises the development of a regeneration and transformation system for carnation (Dianthus caryophyllus L.) [1]. One of the more critical factors in the genetic transformation is the ability to regenerate a mature plant from a single cell. Therefore much attention was paid to shoot regeneration of carnation. Explants from leaves were used and adventitious shoot formation was developed at the bases of the explants. It was possible to generate adventitious shoots at the transition region from stem to leaf: the leaf base. The position of leaves on the plant appeared to be important for subsequent regeneration. The youngest leaves (leaf position 1) showed the highest regeneration percentage. The shoot regeneration procedure was efficient, 65 % of the explants showed regeneration with an average of 10 shoots per explant. Regenerants from leaf explants did not show aberrant plant growth. The shoot regeneration procedures were applicable for a wide range of cultivars.
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© 1997 Springer Science+Business Media Dordrecht
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Van Altvorst, A.C., Bovy, A.G., Angenent, G.C., Dons, J.J.M. (1997). Genetic Modification of Ethylene Biosynthesis and Ethylene Sensitivity in Carnation. In: Kanellis, A.K., Chang, C., Kende, H., Grierson, D. (eds) Biology and Biotechnology of the Plant Hormone Ethylene. NATO ASI Series, vol 34. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5546-5_41
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DOI: https://doi.org/10.1007/978-94-011-5546-5_41
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