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On 1-Aminocyclopropane-1-Carboxylic Acid (ACC) Oxidase

Degradation of a-Aminoisobutyric Acid and Structure-Function Studies on the CO2 Binding Site

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Biology and Biotechnology of the Plant Hormone Ethylene

Part of the book series: NATO ASI Series ((ASHT,volume 34))

Abstract

ACC oxidase catalyzes a complex reaction to form the gaseous plant hormone ethylene as shown by the following reaction equation: ACC + O2 + ascorbate ? C2H4 + HCN + CO2 + dehydroascorbate + 2H2O [1]. The reaction requires the presence of the cofactors Fe2+ [16] and CO2 [1,3]. Although the exact reaction mechanism is still unknown, it is believed that the enzymatic reaction proceeds via the intermediate N-hydroxyl-ACC, which is presumably so unstable that it fragments into C2H4 (derived from C-2,3 of ACC) and cyanoformic acid, the latter being further degraded into HCN (derived from C-1 of ACC) and CO2 (derived from the carboxyl group of ACC). The notion that ACO is a hydroxylase is supported by the amino acid sequence homology shared between ACO and a number of known hydroxylase [4] as well as by the requirement of the enzyme for molecular oxygen, Fe2+, and ascorbate for catalysis.

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References

  1. Dong, J. G., Fernandez-Maculet, J. C, and Yang, S. F. (1992) Purification and characterization of 1-aminocyclopropane-1-carboxylic acid oxidase from ripe apple fruit, Proc. Natl. Acad. Sci. USA 89, 9789–9793.

    Article  PubMed  CAS  Google Scholar 

  2. Dong J. G., Olson, D., Silverstone A., and Yang, S. F. (1992) Sequence of a cDNA for a 1-aminocyclopropane-1-carboxylate oxidase homolog from apple fruit, Plant Physiol. 98, 1530–1531.

    Article  PubMed  CAS  Google Scholar 

  3. Fernandez-Maculet, J. C, Dong J. G., and Yang, S. F. (1993) Activation of 1-aminocyclopropane-1-carboxylate oxidase by carbon dioxide, Biochem. Biophys. Res. Commun. 193, 1168–1173.

    Article  PubMed  CAS  Google Scholar 

  4. Hamilton, A J., Lycett G. W., and Grierson, D. (1990) Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plants, Nature 346, 284–287.

    Article  CAS  Google Scholar 

  5. Hoffiman N. E., Yang S. F., Ichiara, A, and Sakamura, S. (1982) Stereospecific conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene by plant tissues. Conversion of stereoisomers of 1-amino-2-ethylcyclopropane-l-carboxylic acid to 1-butene, Plant Physiol 70, 195–199.

    Article  Google Scholar 

  6. Hyodo, H. and Nishino, T. (1981) Wound-induced ethylene formation in albedo tissue of citrus fruit, Plant Physiol. 67, 421–423.

    Article  PubMed  CAS  Google Scholar 

  7. Kunkel, T. A (1985) Rapid and efficient site-specific mutagenesis without phenotypic selection., Proc. Natl Acad. Sci. USA 82, 488–492.

    Article  PubMed  CAS  Google Scholar 

  8. Liu Y., Su L. Y., and Yang, S. F. (1984) Metabolism of a-aminoisobutyric acid in mung bean hypocotyls in relation to metabolism of 1-aminocyclopropane-1-carboxylic acid, Planta 161, 439–443.

    Article  CAS  Google Scholar 

  9. Lorimer, G. H. and Miziorko, H. M. (1980) Carbamate formation on the e-amino group of a lysyl residue as the basis for the activation of ribulosebisphosphate carboxylase by CO2 and Mg2+, Biochemistry 19, 5328–5334.

    Article  Google Scholar 

  10. Lürssen K., Naumann K., and Schröder, R. (1979) 1-Aminocyclopropane-1-carboxylic acid. An intermediate of the ethylene biosynthesis in higher plants, Z. Pflanzenphysiol 92, 285–294.

    Google Scholar 

  11. Morrow J. S., Keim P., and Gurd, F. R. N. (1974) CO2 adducts of certain amino acids, peptides, and sperm whale myoglobin studied by carbon 13 and proton nuclear magnetic resonance, J. Biol. Chem. 249, 7484–7494.

    PubMed  CAS  Google Scholar 

  12. Neelakantan, L. and Hartung, W.H. (1958) α-Hydroxylamino nitriles and a-hydroxylamino acids, J. Org. Chem. 23, 964–967.

    Article  CAS  Google Scholar 

  13. Satoh, S. and Esashi, Y. (1980) α-Aminoisobutyric acid: A probable competitive inhibitor of conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene, Plant Cell Physiol. 21, 939–949.

    CAS  Google Scholar 

  14. Satoh, S. and Esashi, Y. (1980) D-Amino acid-stimulated ethylene production in seed tissues, Planta 149, 64–68.

    Article  CAS  Google Scholar 

  15. Van] Anken, H. C. and Schiphorst, M. E. (1974) A kinetic determination of ammonia in plasma, Clin. Chim.Acta. 56, 151–

    Article  PubMed  CAS  Google Scholar 

  16. Ververidis, P. and John, P. (1991) Complete recovery in vitro of ethylene-forming enzyme activity, Phytochemistry 30, 725–727.

    Article  CAS  Google Scholar 

  17. Winterbach, H. E. K. and Apps, P. J. (1991) A gas-chromatographic headspace method for the determination of acetone in bovine milk, blood and urine, Onderstepoort J. Vet. Res. 58, 75–79.

    PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan, ROC

    Y.-y. Charng & S. F. Yang

  2. Department of Vegetable Crops, University of California, Davis, California, 95616, USA

    Y.-y. Charng, Y. Liu, J. G. Dong & S. F. Yang

Authors
  1. Y.-y. Charng
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  2. Y. Liu
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  3. J. G. Dong
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  4. S. F. Yang
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Editor information

Editors and Affiliations

  1. Institute of Viticulture Vegetable Crops & Floriculture, National Agricultural Research Foundation, Heraklion, Crete, Greece

    A. K. Kanellis

  2. Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology — Hellas, Heraklion, Crete, Greece

    A. K. Kanellis

  3. Department of Plant Biology, University of Maryland, College Park, MD, USA

    C. Chang

  4. MSU-DOE, Plant Research Laboratory, Michigan State University, East Lansing, MI, USA

    H. Kende

  5. BBSRC Research Group in Plant Gene Regulation, Department of Physiology and Environmental Science, University of Nottingham, Loughborough, UK

    D. Grierson

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© 1997 Springer Science+Business Media Dordrecht

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Charng, Yy., Liu, Y., Dong, J.G., Yang, S.F. (1997). On 1-Aminocyclopropane-1-Carboxylic Acid (ACC) Oxidase. In: Kanellis, A.K., Chang, C., Kende, H., Grierson, D. (eds) Biology and Biotechnology of the Plant Hormone Ethylene. NATO ASI Series, vol 34. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5546-5_4

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  • DOI: https://doi.org/10.1007/978-94-011-5546-5_4

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6336-4

  • Online ISBN: 978-94-011-5546-5

  • eBook Packages: Springer Book Archive

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