Skip to main content

An Ethylene-Regulated DNA Element in Abscission-Specific Gene Promoters and the Expression of an ETR1 Homologue in Tomato Abscission

  • Chapter
Biology and Biotechnology of the Plant Hormone Ethylene

Part of the book series: NATO ASI Series ((ASHT,volume 34))

  • 424 Accesses

Abstract

Abscission is evoked in response to both environmental and developmental cues, e.g., fruit ripening, senescence, disease, insect damage, fertilization, drought, heat, etc. Both the quality and yield of a crop can be affected by the number of flowers and young fruit that abscise prematurely. An understanding of the biology of abscission is of both commercial and academic interest. The intent of this article is to review progress in my laboratory towards an understanding of the hormonal and cell-specific regulation of abscission.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Brown, S.M. and Crouch, M.L. (1990) Characterization of a family abundantly expressed in Oenothera organesis pollen that shows sequence similarity to polygalacturonase. Plant Cell 2, 263–274.

    PubMed  CAS  Google Scholar 

  2. Chalfie M., Tu Y., Euskirchen G., Ward W.W., and Prasher, D.C. (1994) Green Fluorescent Protein as a marker for gene expression. Science 263, 802–805.

    Article  PubMed  CAS  Google Scholar 

  3. Chang, C, Kwok, S.F., Bleecker, A.B., and Meyerowitz, E.M. (1993) Arabidopsis ethylene-response gene ETR1: Similarity of product to two-component regulators. Science 262, 539–544.

    Article  PubMed  CAS  Google Scholar 

  4. Delia Penna D., Alexander, D.C, and Bennett, A.B. (1986) Molecular cloning of tomato fruit polygalacturonase: Analysis of polygalacturonase mRNA levels during ripening. Proc Natl Acad Sci USA 83, 6420–6424.

    Article  Google Scholar 

  5. Durbin, M.L., Sexton R., and Lewis, L.N. (1981) The use of immunological methods to study the activity of cellulase isozymes (β-1,4-glucan 4-glucan hydrolase) in bean leaf abscission. Plant Cell Environ 4, 67–73.

    Article  CAS  Google Scholar 

  6. Hua J., Chang, C, Sun Q., and Meyerowitz, E.M. (1995) Ethylene insensitivity conferred by Arabidopsis ERS gene. Science 269, 1712–1714.

    Article  PubMed  CAS  Google Scholar 

  7. Jackson, M.B. and Osborne, D.J. (1970) Ethylene, the natural regulator of leaf abscission. Nature 225, 1019–1022.

    Article  PubMed  CAS  Google Scholar 

  8. Jefferson, R.A., Kavanagh, T.A., and Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO 6, 3901–390

    CAS  Google Scholar 

  9. Kalaitzis P., Koehler, S.M., and Tucker, M.L. (1995) Cloning of a tomato polygalacturonase expressed in abscission. Plant Mol Biol 28, 647–656.

    Article  PubMed  CAS  Google Scholar 

  10. Kay R., Chan A., Daly M., and McPherson, J. (1987) Duplication of CaMV 35S promoter sequences creates a strong enhancer for plant genes. Science 236, 1299–1302.

    Article  PubMed  CAS  Google Scholar 

  11. Kemmerer, E.C. and Tucker, M.L. (1994) Comparative study of cellulases associated with adventitious root initiation, apical buds, and leaf, flower, and pod abscission zones in soybean. Plant Physiol 104, 557–562.

    Article  PubMed  CAS  Google Scholar 

  12. Koehler, S.M., Matters, G.L., Nath P., Kemmerer, E.C., and Tucker, M.L. (1996) The gene promoter for a bean abscission cellulase is ethylene-induced in transgenic tomato and shows high sequence conservation with a soybean abscission cellulase. Plant Mol Biol (in press)

    Google Scholar 

  13. Koehler, D.E., Lewis, L.N., Shannon, L.M., and Durbin, M.L. (1981) Purification of a cellulase from Kidney bean abscission zones. Phytochemistry 20, 409–412.

    Article  CAS  Google Scholar 

  14. Lewis, L.N. and Varner, J.E. (1970) Synthesis of cellulase during abscission of Phaseolus vulgaris leaf explants. Plant Physiol 46, 194–199.

    Article  PubMed  CAS  Google Scholar 

  15. Milligan, S.B. and Gasser, C.S. (1995) Nature and regulation of pistil-expressed genes in tomato. Plant Mol Biol 28, 691–711.

    Article  PubMed  CAS  Google Scholar 

  16. Neelam, A. and Sexton, R. (1995) Cellulase (endo β-1,4 glucanase) and cell wall breakdown during anther development in the Sweet Pea (Lathyrus odoratus L.): Isolation and characterization of partial cDNA clones. J Plant Physiol 146, 622–628.

    Article  CAS  Google Scholar 

  17. Osborne, D.J. (1989) Abscission. Crit Rev Plant Sri 8, 103–129.

    Article  CAS  Google Scholar 

  18. Ow, D.W., Wood, K.V., De Luca M., De Wet, J.R., Helinski, D.R., and Howell, S.H. (1986) Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Science 234, 856–859.

    Article  PubMed  CAS  Google Scholar 

  19. Schaller, G.E. and Bleecker, A.B. (1995) Ethylene binding sites generated in yeast expressing the Arabidopsis ETR1 gene. Science 270, 1809–1811.

    Article  PubMed  CAS  Google Scholar 

  20. Sexton R., Durbin, M.L., Lewis, L.N., and Thomson, W.W. (1981) The immunocytochemical localization of 9.5 cellulase in abscission zones of bean (Phaseolus vulgaris cv Red Kidney). Protoplasma 109, 335–347.

    Article  CAS  Google Scholar 

  21. Tucker, M.L., Sexton R., del Campillo E., and Lewis, L.N. (1988) Bean abscission cellulase: Characterization of a cDNA clone and regulation of gene expression by ethylene and auxin. Plant Physiol 88, 1257–1262.

    Article  PubMed  CAS  Google Scholar 

  22. Tucker, M.L., Baird, S.L., and Sexton, R. (1991) Bean leaf abscission: Tissue specific accumulation of a cellulase mRNA. Planta 186, 52–57.

    Article  CAS  Google Scholar 

  23. Tucker, M.L., Matters G.L., Koehler, S.M., Kemmerer, E.C., and Baird S.L. (1993) Hormonal and tissue-specific regulation of cellulase gene expression in abscission. In: Pech JC, Latche A, Balague C (eds), Cellular and Molecular Aspects of the Plant Hormone Ethylene, Current Plant Science and Biotechnology in Agriculture, Vol 16 Kluwer Academic Publishers, Boston, pp. 265–271.

    Google Scholar 

  24. Wilkinson, J.Q., Lanahan, M.B., Yen, H-C., Giovannoni, J.J., and Klee, H.J. (1995) An ethylene-inducible component of signal transduction encoded by Never-ripe. Science 270, 1807–1809.

    Article  PubMed  CAS  Google Scholar 

  25. Zhou D., Kalaitzis P., Mattoo, A.K., and Tucker ML (1996) The mRNA for an ETR1 homologue in tomato is constitutively expressed in vegetative and reproductive tissues. Plant Mol Biol 30, 1331–1338.

    Article  PubMed  CAS  Google Scholar 

  26. Zhou D., Mattoo, A.K., and Tucker, M.L. (1996) Molecular cloning of a tomato cDNA (accession no. U47279) encoding an ethylene receptor. Plant Physiol 110, 1435–1436.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1997 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Tucker, M.L. et al. (1997). An Ethylene-Regulated DNA Element in Abscission-Specific Gene Promoters and the Expression of an ETR1 Homologue in Tomato Abscission. In: Kanellis, A.K., Chang, C., Kende, H., Grierson, D. (eds) Biology and Biotechnology of the Plant Hormone Ethylene. NATO ASI Series, vol 34. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5546-5_23

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-5546-5_23

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6336-4

  • Online ISBN: 978-94-011-5546-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics