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Cloning and Physical Characterization of the tox-argK Gene Cluster from Pseudomonas syringae pv. phaseolicola, and Phaseolotoxin Production in Escherichia coli

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Pseudomonas Syringae Pathovars and Related Pathogens

Part of the book series: Developments in Plant Pathology ((DIPP,volume 9))

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Abstract

The bacterium Pseudomonas syringae pv. phaseolicola (P.s. pv, phaseolicola), is the only proven producer of phaseoiotoxin, a potent inhibitor of the enzyme ornithine carbamoyltransferase (OCTase). Phaseoiotoxin production and autoimmunity are genetically controlled by the chromosomal tox-argK gene cluster. To better characterize the physical structure, functional organization, and phylogenetic origin of this cluster, (f) we determined the location of its structural borders in reference to Pseudomonas syringae pv. giycinea’s genome, and (H) cloned a 38 Kb Kpnl chromosomal fragment that contains the entire cluster plus adjacent regions and enables Escherichia coli (E. coli) to produce phaseoiotoxin.

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Author information

Authors and Affiliations

  1. USDA-ARS, Foreign Disease-Weed Science Research Unit, Frederick, MD, 21702, USA

    Efstathios Hatziloukas & Yuichi Takikawa

  2. Faculty of Agriculture, Shizuoka University, 836, Ohya, Shizuoka, 422, Japan

    Norman Schaad

  3. Institute Molecuiar Biology & Biotechnology, F.O.R.T.H., and Dept. of Biology, University of Crete, P.O. Box 2208, Heraklion, 71409, Crete, Greece

    Nickolas Panopoulos

Authors
  1. Efstathios Hatziloukas
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  2. Yuichi Takikawa
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  3. Norman Schaad
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  4. Nickolas Panopoulos
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Editor information

Editors and Affiliations

  1. Institut für Pflanzenpathologie und Pflanzenschutz der Universität, Göttingen, Germany

    K. Rudolph

  2. Department of Plant Pathology, NYSAES-Cornell University, Geneva, NY, USA

    T. J. Burr

  3. Department Biological Sciences, Wye College, University of London, Wye, Ashford, Kent, UK

    J. W. Mansfield

  4. Central Science Laboratory, Sand Hutton, York, UK

    D. Stead

  5. Department of Biological Science, UWE-Bristol, Frenchay, Bristol, UK

    A. Vivian

  6. Biologische Bundesanstalt für Land- und Forstwirtschaft, Braunschweig, Germany

    J. von Kietzell

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© 1997 Springer Science+Business Media Dordrecht

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Hatziloukas, E., Takikawa, Y., Schaad, N., Panopoulos, N. (1997). Cloning and Physical Characterization of the tox-argK Gene Cluster from Pseudomonas syringae pv. phaseolicola, and Phaseolotoxin Production in Escherichia coli . In: Rudolph, K., Burr, T.J., Mansfield, J.W., Stead, D., Vivian, A., von Kietzell, J. (eds) Pseudomonas Syringae Pathovars and Related Pathogens. Developments in Plant Pathology, vol 9. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5472-7_44

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  • DOI: https://doi.org/10.1007/978-94-011-5472-7_44

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6301-2

  • Online ISBN: 978-94-011-5472-7

  • eBook Packages: Springer Book Archive

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