Abstract
The species description of Cambarincola holti was emended to include variations in appearance of the peristomium, a description of the jaws, the shape of a normal spermatheca, and the correct description of the glandular atrium, granular rather than vacuolar. The glandular atrium contained a single type of secretion granule characterized as mucoprotein or glycoprotein, with an alkaline protein component. The vacuolar prostate gland secretions are uncharacterized as no stains would attach to the material. Spermatozoa were sometimes found in the spermathecal duct, but usually they were clumped together in the spermathecal bulb, or randomly distributed with each acrosome being in contact with a lining cell. The digitiform, glandular atrium of Xironogiton victoriensis is composed of nine regions based on the staining reaction of the granules. The granules were characterized as neutral mucoprotein, nonsulfated acid mucosubstance, glycoproteins, a combination of sulfated and nonsulfated acid mucosubstances, and totally non-staining granules. Spermatophores were observed only in X. victoriensis in depressions on the clitellum. The spermatozoa were separated from the cuticle by a layer of PAS staining material and covered by a lighter, PAS staining cap with a foamy outer layer that stains lightly with AB2.5. Further studies to characterize the secretions of the male reproductive organs may produce an additional character to add to the data matrices for phylogenetic analyses of these clitellates.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Brinkhurst, R. O. & S. R. Gelder, 1991. Chapter 12. Annelida: Oligochaeta and Branchiobdellida. In: J. H. Thorpe & F. Covitch (eds), Ecology and Classification of North American Freshwater Invertebrates. Academic Press, New York: 401–435.
Erséus, C, 1993. The marine Tubificidae (Oligochaeta) of Rottnest Island, Western Australia. In: F. E. Wells, D. I. Walker, H. Kirkman & R. Lethbridge (eds), Proceedings of the Fifth International Marine Biological Workshop: The Marine Flora and Fauna of Rottnest Island, Western Australia. Western Australian Museum, Perth: 331–390.
Ferraguti, M. & S. R. Gelder, 1991. The comparative ultrastructure of spermatozoa from five branchiobdellidans (Annelida: Clitellata). Can. J. Zool. 69: 1945–1956.
Gelder, S. R. & R. O. Brinkhurst, 1990. An assessment of the phytogeny of the Branchiobdellida (Annelida: Clitellata) using RAUP. Can. J. Zool. 68: 1318–1326.
Gelder, S. R. & L. A. Hall, 1990. Branchiobdellidans from southwestern British Columbia, Canada with a phylogenetic analysis of Xironogiton (Annelida: Clitellata) using PAUP. Can. J. Zool. 68: 2352–2359.
Gelder, S. R., M. Ferraguti & M. A. Subchev, 1994. A description of spermatozoan ultrastructure and some anatomical characters in Branchiobdella kozarovi Subchev, 1978 (Annelida: Clitellata), and a review of the spermatozoan morphology within the genus. Hydrobiologia 278: 17–26.
Goodnight, C. J., 1943. Report on a collection of branchiobdellids. J. Parasitol. 29: 100–102.
Henle, J. F. G., 1835. Über die Gattung Branchiobdella. Arch. Anat. Physiol, wiss. Medizin: 574–608.
Hobbs, H. H., Jr., 1989. An illustrated checklist of the American crayfishes (Decapoda: Astacidae, Cambaridae, and Parastacidae). Smithson. Contr. Zool. 480: 236 pp.
Hoffman, R. L., 1963. A revision of the North American annelid worms of the genus Cambarincola (Oligochaeta: Branchiobdell-idae). Proc. US nat. Mus. 114: 271–371.
Holt, P. C, 1949. A comparative study of the reproductive systems of Xironogiton instabilis (Moore) and Cambarincola philadelphica (Leidy) (Annelida, Oligochaeta, Branchiobdellidae). J. Morph. 84: 535–572.
Holt, P. C, 1960. The genus Ceratodrilus Hall (Branchiobdellidae, Oligochaeta) with the description of a new species. Virginia J. Sci. 11:53–77.
Holt, P. C., 1974. The genus Xironogiton Ellis, 1919 (Clitellata: Branchiobdellida). Virginia J. Sci. 25: 5–19.
Holt, P. C., 1981. A resume of the members of the genus Cambarincola (Annelida: Branchiobdellida) from the Pacific drainage of the United States. Proc. biol. Soc. Wash. 94: 675–695.
Holt, P. C., 1986. Newly established families of the order Branchiobdellida (Annelida: Clitellata) with a synopsis of the genera. Proc. biol. Soc. Wash. 99: 676–702.
Holt, P. C. & B. D. Opell, 1993. A checklist of and illustrated key to the genera and species of the central and North American Cambarincolidae (Clitellata: Branchiobdellida). Proc. biol. Soc. Wash. 106: 251–295.
Humason, G. L., 1979. Animal Tissue Techniques. 4th ed. Freeman and Co., San Francisco, 661 pp.
James, J. & J. Tass, 1984. Histochemical Protein Staining Methods. Oxford Univ. Press, Oxford, U.K., 40 pp.
Jamieson, B. G. M., 1981. The Ultrastructure of the Oligochaeta. Academic Press, New York, 462 pp.
Jamieson, B. G. M., 1992. Chapter 3. Oligochaeta. In: F. W. Harrison & S. L. Gardiner (eds), Microscopical Anatomy of Invertebrates, Volume 7: Annelida. Wiley-Liss, New York: 301–316.
Sawyer, R. T., 1986. Leech Biology and Behaviour. Clarendon Press, Oxford, 1065 pp.
Schroeder, P. C. & C. O. Hermans, 1975. Annelida: Polychaeta. In: A. C. Giese & J. S. Pearse (eds), Reproduction of Marine Invertebrates. Academic Press, New York, Vol. 3:1–213.
Sheehan, D. C. & B. B. Hrapchak, 1980. Theory and practice of Histotechnology. C. V. Morsby Co., St. Louis, 441 pp.
Yamaguchi, H., 1934. Studies on Japanese Branchiobdellidae with some revisions on the classification. J. Fac. Sci. Hokkaido Imp. Un. Ser. VI. (Zool.) 3: 177–219.
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1996 Springer Science+Business Media Dordrecht
About this paper
Cite this paper
Gelder, S.R. (1996). Histochemical characterization of secretions in the reproductive systems of two species of branchiobdellidans (Annelida: Clitellata): a new character for the phylogenetic matrix?. In: Coates, K.A., Reynoldson, T.B., Reynoldson, T.B. (eds) Aquatic Oligochaete Biology VI. Developments in Hydrobiology, vol 115. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5452-9_25
Download citation
DOI: https://doi.org/10.1007/978-94-011-5452-9_25
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6293-0
Online ISBN: 978-94-011-5452-9
eBook Packages: Springer Book Archive