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Enzyme-linked immunosorbant assay in the rheumatological laboratory

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Manual of Biological Markers of Disease

Abstract

The increasing popularity of enzyme-linked immunosorbant assay (ELISA) for the detection of autoantibodies of value in the diagnosis of rheumatological diseases has led to the development of assay systems which are proving to be of great utility. Unfortunately, in practice, the results do not always match the high standards which are appropriate for use in routine diagnosis or application in research. In this paper we offer guidelines on practical aspects of employing ELISA to detect autoantibodies and describe methods and an approach which should assure a good quality of result.

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Abbreviations

OD:

optical density

ELISA:

enzyme-linked immunosorbent assay

DIB:

dot immunobinding assay

CIE:

counterimmuno electrophoresis

OPD:

orthophenylene diamine

TMB:

3,3’,5,5’-tetramethyl-benzidine

ABTS:

2,2’-azino-dia(3’-ethylbenzthiazoline-6-sulfonic acid)

ONPG:

ortho-nitrophenyl-B-D-galactosidase

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Author information

Authors and Affiliations

  1. Immunology and Immunogenetics Laboratory, Department of Rheumatology, Charing Cross Hospital, and Kennedy Institute of Rheumatology, London, W6 8RF, UK

    P. J. Charles & R. N. Maini

Authors
  1. P. J. Charles
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  2. R. N. Maini
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Editor information

Editors and Affiliations

  1. University of Nijmegen, The Netherlands

    W. J. Van Venrooij

  2. The Mathilda and Terence Kennedy Institute of Rheumatology, London, UK

    R. N. Maini

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© 1993 Springer Science+Business Media Dordrecht

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Charles, P.J., Maini, R.N. (1993). Enzyme-linked immunosorbant assay in the rheumatological laboratory. In: Van Venrooij, W.J., Maini, R.N. (eds) Manual of Biological Markers of Disease. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5444-4_5

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  • DOI: https://doi.org/10.1007/978-94-011-5444-4_5

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-4243-4

  • Online ISBN: 978-94-011-5444-4

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