Abstract
The detection of autoantibodies by indirect immunofluorescent screening techniques has been supplemented by laboratory tests that characterise individual reactions, thereby providing clinicians with important diagnostic and prognositic information [1]. Counterimmunoelectrophoresis (CIE) and double diffusion (Ouchterlony) are two of the methods available for identifying specific antibodies in complex antigen mixtures [2, 3] and have found particular application with respect to the detection of antibodies to soluble cellular antigens, sometimes referred to as extractable nuclear antigens (ENA).
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Abbreviations
- CIE:
-
counterimmunoelectrophoresis
- EEO:
-
electroendosmosis
- ENA:
-
extractable nuclear antigen
- ID:
-
immunodiffusion
- PBS:
-
phosphate buffered saline
- HIV:
-
human immunodeficiency virus
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© 1993 Springer Science+Business Media Dordrecht
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Bunn, C., Kveder, T. (1993). Counterimmunoelectrophoresis and immunodiffusion for the detection of antibodies to soluble cellular antigens. In: Van Venrooij, W.J., Maini, R.N. (eds) Manual of Biological Markers of Disease. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5444-4_3
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DOI: https://doi.org/10.1007/978-94-011-5444-4_3
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