Experimental and theoretical analysis of cell separations by ultrasonic forces

Abstract

The ultrasonic separation of mammalian cells has been successfully used for cell retention in long-term perfusion processes [1, 2]. Ultrasonic separators are free of the problems typically encountered with conventional cell separation systems such as filter fouling, long sedimentation times or complex centrifuge operation. Acoustic separation technology relies on the aggregation of cells by ultrasonic forces in a standing wave field. In this work, a versatile method is presented for the experimental measurement of the forces on single particles as a function of position in the standing wave field. A detailed understanding of the forces should accelerate the rational design of acoustic separation devices.