Abstract
In this study, organic contaminants present in Pluronic F-68 were extracted using Supercritical Fluid Extraction (SFE). Combined Gas Chromatography-Mass Spectrophotometry analyses of extracted components of Pluronic F-68 (obtained from two commercial suppliers) were shown to contain butylated hydroxy toluene (BHT) antioxidant and phthalate plasticizer. Both sources of Pluronic F-68 polymers and their SFE components were shown to be cytotoxic to LL24 human lung fibroblast cells. Cells which were cultivated in unpurified Pluronic F-68 were observed to have a large number of vacuoles and lipid or polymer-loaded vesicles, as evidenced by Transmission Electron Microscopy (TEM). In medium supplemented with SFE extracts of Pluronic, the LL24 cells had significantly reduced viability and cell size and increased lipid droplet formation. We conclude from these findings that contaminating molecules (such as phthalates and BHT) in Pluronic F-68 are cytotoxic and may cause impairment of lipid catabolism.
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© 1997 Springer Science+Business Media Dordrecht
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Handa-Corrigan, A., Weeratunge, N.P., Braybrook, J.H., Mackay, G.A., Chescoe, D. (1997). In-Vitro Toxicological Effects of Pluronic F-68. In: Carrondo, M.J.T., Griffiths, B., Moreira, J.L.P. (eds) Animal Cell Technology. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5404-8_20
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DOI: https://doi.org/10.1007/978-94-011-5404-8_20
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