Abstract
Porcine brain microvascular endothelial cells (PBMEC) were transfected with pRNS-1, encoding for the small and large T-antigens of SV 40, by means of lipofection. After selection with G-418, clones were isolated and one clone, PBMEC/C1-2 was further characterized. PBMEC/C1-2 was up to now cultivated for nearly one year with 250 cumulative population doublings and showed the typical morphology of capillary endothelial cells in vitro. SV 40 T-antigens were present in the nuclei, the cells showed the typical granular staining of von Willebrand factor (vWF) and were positive for Bandeiraea simplicifolia isolectin B4. Furthermore, PBMEC/C1-2 exhibited expression of non-muscular and smooth-muscle type myosins, and the presence of the blood-brain barrier-associated markers γ-glutamyltranspeptidase (y-GT), the glucose transporter Glut-1 and apolipoprotein A-1 (Apo A-1). In addition, enzymatic activity of y-GT and alkaline Phosphatase (ALP) was detected in cell suspensions. In summary, PBMEC/C1-2 represent an immortalized PBMEC line exhibiting endothelial characteristics as well as typical markers of the blood-brain barrier.
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© 1997 Springer Science+Business Media Dordrecht
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Teifel, M., Friedl, P. (1997). Immortalization and Characterization of Porcine Brain Microcapillary Endothelial Cells. In: Carrondo, M.J.T., Griffiths, B., Moreira, J.L.P. (eds) Animal Cell Technology. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5404-8_18
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DOI: https://doi.org/10.1007/978-94-011-5404-8_18
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