Abstract
Mice primary brain cell cultures can be used as model systems to study biochemical processes in the brain. As the brain consists of a complex mixture of different cell types, using NMR (Nuclear Magnetic Resonance) spectroscopy and suitable cell culturing techniques, it is possible to study cell metabolism of each cell type separately or in defined co-cultures.
The aim of this work was the development of a suitable perfusion system to keep primary brain cells under physiological conditions for long periods in the NMR tube, without losses in viability.
Immobilization of astrocytes and neurons in three-dimensional structures is described. These immobilization techniques allowed high cell densities and protection from shear stress due to perfusion flow. Their feasibility for perfusion in the spectrometer is demonstrated using 31P-NMR spectra to monitor cell energy state and thus well-being of the cells.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Cox DWG, Morris PG, Feeney J & Bachelard HS (1983) 31P-n.m.r. Studies on Cerebral Energy Metabolism under Conditions of Hypoglycaemia and Hypoxia in vitro. Biochem. J. 212, 365–370.
Brand A, Engelmann J & Leibfritz D (1992) A 13C NMR Study on Fluxes into TCA Cycle of Neuronal and Glial cell line and Primary Cells. Biochimie 74: 941–948.
Sonnewald U, Westergaard N, Schousboe A, Svendson JS, Unsgard G & Petersen SB (1993) Direct Demonstration by [13C]NMRspectroscopy that Glutamine from Astrocytes is a Precursor for GABA synthesis in Neurons. Neurochem. Int. 22: 19–29.
Alves PM, Flögel U, Brand A, Leibfritz D, Carrondo MJT, Santos H, Sonnewald U (1996) Immobilization of Primsary Astrocytes and Neurons for On-Line Monitoring of Biochemical Processes by NMR. Dev. Neurosci., in press.
Schousboe A, Meier E, Drejer J & Hertz L (1989) Preparation of Primary Cultures of Mouse (rat) Cerebellar Granule Cells. In: Shahar A, De Vellis J, Vernadakis A & Haber B (eds), A Dissecation and Tissue Culture Manual of the Nervous System, pp 203–206. Alan R. Liss, New York, EUA.
Hertz L, Juurlink BHJ, Hertz EH, Fosmark H. & Schousboe A (1989) Preparation of primary cultures of mouse (rat) astrocytes. In: Shahar A, De Vellis J, Vernadakis A & Haber B (eds), A Dissecation and Tissue Culture Manual of the Nervous System, pp 105–108. Alan R. Liss, New York, EUA.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1997 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Alves, P.M., Carrondo, M.J.T., Santos, H. (1997). Immobilization of Primary Brain Cells in Porous Microcarriers for on Line NMR Spectroscopy. In: Carrondo, M.J.T., Griffiths, B., Moreira, J.L.P. (eds) Animal Cell Technology. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5404-8_15
Download citation
DOI: https://doi.org/10.1007/978-94-011-5404-8_15
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6273-2
Online ISBN: 978-94-011-5404-8
eBook Packages: Springer Book Archive