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The Effect of Intragastric Capsaicin and Resiniferatoxin on Indomethacin- Induced Gastric Mucosal Damage in Rats

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Cell Injury and Protection in the Gastrointestinal Tract

Abstract

Capsaicin-sensitive sensory nerves have local protective functions in the stomach. In the present study, the effect of capsaicin or its analogue, resiniferatoxin (RTX), on gastric mucosal injury caused by indomethacin was studied in pylorus-ligated rats. Gastric mucosal damage was evoked by the subcutaneous (sc) or intragastric (ig) administration of indomethacin (20 mg/kg) together with the ig administration of 2 ml of physiological saline or 2 ml of 0.15 N HCI. Animals received simultaneously capsaicin or RTX ig at different concentrations and were sacrificed 4 h later. Resiniferatoxin administered ig at a low concentration (40 ng/ml; 0.4 μg/kg) almost totally prevented the development of gastric mucosal injury by sc or ig indomethacin plus ig saline. The protective effect of RTX was not modified by atropine (0.1 mg/kg) or cimetidine (10 mg/kg) treatment, but was not evident after bilateral subdiaphragmatic vagotomy. The sc administration of indomethacin together with ig application of 0.15 N HCl resulted in much more severe gastric mucosal damage than that seen after indomethacin and ig saline. Capsaicin or RTX (both applied at 60 and 120 ng/m1) protected against gastric mucosal injury produced by indomethacin plus ig HCl in a concentration-dependent manner. Data indicate that acid in the stomach has a potentiating effect on the development of the indomethacin-induced gastric injury and that ig capsaicin or RTX at very low concentrations protect against the indomethacin- or indomethacin-plus-acid-induced gastric mucosal injury in rats.

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Authors and Affiliations

  1. First Department of Medicine, Medical University of Pécs, Ifjúság út, H-7643, Pécs, Hungary

    O. M. E. Abdel Salam & Gy. Mózsik

  2. Department of Pharmacology, Medical University of Pécs, Ifjúság út, H-7643, Pécs, Hungary

    J. Szolcsányi

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  1. O. M. E. Abdel Salam
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  2. J. Szolcsányi
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  3. Gy. Mózsik
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Editors and Affiliations

  1. Pécs, Hungary

    Gy. Mózsik , L. Nagy  & A. Pár ,  & 

  2. Sheffield, UK

    K. D. Rainsford

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© 1997 Springer Science+Business Media Dordrecht

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Salam, O.M.E.A., Szolcsányi, J., Mózsik, G. (1997). The Effect of Intragastric Capsaicin and Resiniferatoxin on Indomethacin- Induced Gastric Mucosal Damage in Rats. In: Mózsik, G., Nagy, L., Pár, A., Rainsford, K.D. (eds) Cell Injury and Protection in the Gastrointestinal Tract. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5392-8_9

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  • DOI: https://doi.org/10.1007/978-94-011-5392-8_9

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6268-8

  • Online ISBN: 978-94-011-5392-8

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