Abstract
The detailed mechanism of H. pylori-induced gastric damage is still unknown. In this study, we showed that a water extract of H. pylori retarded the epithelial restoration in a cultured rabbit gastric epithelial cell model with the inhibition of both cell migration and proliferation. Although this inhibitory substance has not yet been biochemically characterized, it is different from known H. pylori associatecytotoxins and ammonia. From these results, we hypothesize that these bacteria directly play a role in disturbance of peptic ulcer healing in vivo.
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References
Ito S, Lacy ER, Rutten MJ, Critchow J, Silen W. Rapid repair of injured gastric mucosa. Scand J Gastroenterol. 1984;19(Supp1.101):87–95.
Svanes K, Takeuchi K, Ito S, Silen S. Restitution of the surface epithelium of the in vitro frog gastric mucosa after damage with hyperosmolar sodium chloride. Morphological and physiological characteristics. Gastroenterology. 1982;83:1409–26.
Silen W, Ito S. Mechanism for rapid reepithelization of the gastric mucosal surface. Annu Rev Physiol. 1985;47:217–19.
Watanabe S, Hirose M, Yasuda T, Sato N. Role of actin and calmodulin in migration and proliferation of rabbit gastric mucosal cells in culture. Gastroenterol Hepatol. 1994;9:325–33.
Watanabe S, Hirose M, Wang XE et al. Hepatocyte growth factor accelerated the wound repair of cultured gastric mucosal cells. Biochem Biophys Res Commun. 1994;199:1453–60.
Watanabe S, Wang XE, Hirose M, Sato N. Effect of myosin light chain kinase inhibitor wortmannin on the wound repair of cultured gastric mucosal cells. Biochem Biophys Res Commun. 1994;199:799–806.
Mikami H, Watanabe S, Hirose M, Sato N. Role of extracellular matrix in wound repair by cultured gastric mucosal cells. Biochem Biophys Res Commun. 1994;202:285–92.
Watanabe S, Wang XE, Hirose M et al. Platelet-derived growth factor accelerates gastric epithelial restoration in a rabbit cultured cell model. Gastroenterology. 1996;110:775–9.
Axon AH. H. pylori and gastroduodenal disease. Practitioner. 1991;235:733–6.
Blaser MJ. Hypothesis on the pathogenesis and natural history of Helicobacter pylori-induced inflammation. Gastroenterology. 1992;102:720–7.
Cover TL. Helicobacter pylori and gastroduodenal disease. Annu Rev Med. 1992;43:135–45.
Graham DY. Helicobacter pylori: its epidemiology and its role in duodenal ulcer disease. J Gastroenterol Hepatol. 1991;6:97–105.
Megraud F, Lamouliatte H. Helicobacter pylori and duodenal ulcer: evidence suggesting causation. Dig Dis Sci. 1992;37:769–72.
Megraud F, Neman-Simha V, Brugmann D. Further evidence of the toxic effect of ammonia produced by Helicobacter. Infect Immun. 1992;60:1858–63.
Covacci A, Censini S, Bugnoli M et al. Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc Natl Acad Sci USA. 1993;90:5791–5.
Cover TL. Purification and characterization of vacuolating toxin from Helicobacter pylori. J Biol Chem. 1992;267:10570–5.
Craig PM, Territo MC, Karnes WE, Walsh JH. Helicobacter pylori secretes a chemotactic factor for monocytes and neutrophils. Gut. 1992;33:1020–3.
Graham DY. Pathogenic mechanisms leading to Helicobacter pylori-induced inflammation. Eur J Gastroenterol Hepatol. 1992;4:S9–S16.
Wallace JL. Possible mechanisms and mediators of gastritis associated with Helicobacter pylori infection. Scand J Gastroenterol. 1991;26(Supp1.187):65–70.
Kurose I, Granger DN, Evans DJ Jr et al. Helicobacter pylori-induced microvascular protein leakage in rats: Role of neutrophils, mast cells, and platelets. Gastroenterology. 1994;107:70–9.
Yoshida N, Granger DN, Evans DJ Jr et al. Mechanisms involved in Helicobacter pylori-induced inflammation. Gastroenterology. 1993;105:1431–40.
Tummuru MR, Cover TL, Blaser MJ. Cloning and expression of a high-molecular-mass major antigen of Helicobacter pylori: Evidence of linkage to cytotoxin production. Infect Immun. 1993;61:1799–809.
Mai UEH, Perez-Perez GI, Wahl LM, Wahl SM, Blaser MJ, Smith PD. Soluble surface proteins from Helicobacter pylori activate monocytes/macrophages by lipopolysaccharide-independent mechanism. J Clin Invest. 1991;87:894–900.
Mai UEH, Perez-Perez GI, Allen JB, Wahl SM, Blaser MJ, Smith PD. Surface proteins from Helicobacter pylori exhibit chemotactic activity for human leukocytes and are present in gastric mucosa. J Exp Med. 1992;175:517–25.
Neisen H, Andersen LP. Chemotactic activity of Helicobacter pylori sonicate for human polymorphonuclear leucocytes and monocytes. Gut. 1992;33:738–42.
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© 1997 Springer Science+Business Media Dordrecht
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Sato, N. et al. (1997). Inhibition of Rabbit Gastric Epithelial Restoration by a Water Extract of Helicobacter Pylori: Evidence Using a Cultured Cell Model. In: Mózsik, G., Nagy, L., Pár, A., Rainsford, K.D. (eds) Cell Injury and Protection in the Gastrointestinal Tract. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5392-8_19
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DOI: https://doi.org/10.1007/978-94-011-5392-8_19
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