Abstract
Five subtypes of cholinergic muscarinic receptors (M1–M5) have been cloned and characterized by pharmacological and molecular biological methods during the past decade. The M3 subtype was demonstrated in acid-secreting cells, both by functional measurements and binding assays, while the M1 subtype is reported in the intramural ganglionic cells. In the present study, the distribution of M1–M5 mRNAs was mapped by in-situ hybridization histochemistry. Sections (12-µm thick, frozen) from different parts of the stomachs from control or immobilized (3 h immobilization on two consecutive days) rats were hybridized with [35S]UTP labelled ribonucleotides (400–500 nucleotides each) directed to mRNAs of the M1–M5 subtypes. M1 and M3 mRNAs were present in different locations and in different densities in the rat stomach; however, there was a complete lack in detection of mRNAs of the M2, M4 or M5 subtypes. M1 mRNA was found over the enteric ganglionic cells and over some deep epithelial and tunica propria cells of the prepyloric area. No labelling was seen over the epithelial cells of the fundic mucosa. The distribution of M1 mRNA was not changed by immobilization. M3 mRNA was detected over a portion of parietal cells (immunolabelled using a monoclonal antibody) of fundic mucosa and over smooth muscle cells of the tunica muscularis. A large number of tunica propria cells and some enteric ganglions were also labelled for M3 subtype. Weak labelling was seen over the surface mucous cells. The density of the labelling was increased in stressed rats, particularly over the tunica propria cells. These data complete earlier observations obtained by pharmacological and radio ligand binding assays on Ml and M3 subtypes in the rat stomach and support the existence of a non-epithelial, non-neuronal cell regulatory system in the GI tract.
Correspondence
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Hunyady, B., Mezey, É., Pacak, K., Harta, G., Palkovits, M. (1997). Distribution of Muscarinic Receptor mRNAs in the Stomachs of Normal or Immobilized Rats. In: Gaginella, T.S., Mózsik, G., Rainsford, K.D. (eds) Biochemical Pharmacology as an Approach to Gastrointestinal Disorders. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5390-4_15
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