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Biochemical Pharmacology as an Approach to Gastrointestinal Disorders pp 115–123Cite as

Gastric Motor Effects of Endothelins in the Lower Brainstem of the Rat

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Abstract

To characterize the modulatory effect of endothelin on brainstem control of gastric motor function, endothelin-1 (ET-1) and endоthelin-3 (ET-3) were applied to the surface of the dorsal medulla oblongata in α-chloralose and xylazine anaesthetized, artificially ventilated Sprague—Dawley rats, while intragastric pressure and contractility of the pyloric circular and greater curvature longitudinal muscles as well as blood pressure were monitored. Endothelin-1 and ET-3 equipotently (at the same range of doses) increased intragastric pressure and stimulated contractility of the gastric circular muscle as well as increasing arterial blood pressure. All the gastric effects of endothelins were abolished by bilateral vagotomy at the midcervical level. These results demonstrate that endothelins have vagally mediated gastrointestinal effects in the lower brainstem of the rat and support a role for endothelins in gastrointestinal regulation.

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References

  1. Yanagisawa M, Kurihara H, Kimura S et al. A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature. 1988; 332: 411–15.

    Article  PubMed  CAS  Google Scholar 

  2. Inoue A, Yanagisawa M, Kimura S et al. The human endothelin family: Three structurally and pharmacologically distinct isopeptides predicted by three separate genes. Proc Natl Acad Sci USA. 1989; 86: 2863–7.

    Article  PubMed  CAS  Google Scholar 

  3. Bloch KD, Hong CC, Eddy RL, Shows TB, Quatermouse T. cDNA cloning and chromosomal assignment of the endothelin 2 gene: Vasoactive intestinal contractor peptide is rat endothelin 2. Genomics. 1991; 10: 236–42.

    Article  PubMed  CAS  Google Scholar 

  4. Kohzuki M, Chai SY, Paxinos G et al. Localization and characterization of endothelin receptor binding sites in the rat brain visualized by in vitroautoradiography. Neuroscience. 1991; 42: 245–60.

    Article  PubMed  CAS  Google Scholar 

  5. Kloog Y, Ambar I, Sokolovsky M, Kochva E, Wollberg Z, Bdolah A. Sarafotoxin, a novel vasoconstrictor peptide: Phosphoinositide hydrolysis in rat heart and brain. Science. 1988; 242: 268–70.

    Article  PubMed  CAS  Google Scholar 

  6. Arai H, Honri S, Aramori I, Ohkubo H, Nakanishi S. Cloning and expression of cDNA encoding endothelin receptor. Nature. 1990; 348: 730–2.

    Article  PubMed  CAS  Google Scholar 

  7. Sakurai T, Yanagisawa M, Takuwa Y et al. Cloning of a cDNA encoding a non-isopeptide-selective subtype of the endothelin receptor. Nature. 1990; 348: 732–5.

    Article  PubMed  CAS  Google Scholar 

  8. Chijiwara Y, Okabe H, Akiho H, Harada N, Nawata H. Functional endothelinA receptor on gastric smooth muscle cells. Digestion. 1995; 56: 171–4.

    Article  Google Scholar 

  9. Sakurai T, Yanagisawa M, Masaki T. Molecular characterization of endothelin receptors. Trends Pharmacol Sci. 1992; 13: 103–8.

    Article  PubMed  CAS  Google Scholar 

  10. Wiklund NP, Ohlen A, Cederqvist B. Adrenergic neuromodulation by endothelin in guinea pig pulmonary artery. Neurosci Lett. 1989; 101: 269–73.

    Article  PubMed  CAS  Google Scholar 

  11. Masaki T, Yanagisawa M, Goto K. Physiology and pharmacology of endothelins. Med Res Rev. 1992; 12: 391–421.

    Article  PubMed  CAS  Google Scholar 

  12. Wiklund NP, Wiklund CU, Ohlen A, Gustafsson LE. Cholinergic neuromodulation by endothelin in guinea pig ileum. Neurosci Lett. 1989; 101: 342–6.

    Article  PubMed  CAS  Google Scholar 

  13. Rae GA. Pharmacology of endothelins in the gastrointestinal tract. In: Gaginella TS, ed. Regulatory Mechanisms in Gastrointestinal Function. Boca Raton: CRC Press; 1995: 257–75.

    Google Scholar 

  14. Polak JM, Terenghi G. Distribution of endothelin, a putative growth promoting peptide, using modern microscopical imaging methods. In: Moody TW, ed. Growth Factors, Peptides, and Receptors. New York: Plenum Press; 1993: 3–13.

    Chapter  Google Scholar 

  15. van den Buuse M, Itoh S. Central effects of endothelin on baroreflex of spontaneously hypertensive rats. J Hypertens. 1993; 11: 379–87.

    Article  PubMed  Google Scholar 

  16. Koseki C, Imai M, Nirata Y, Yanagisawa M, Masaki T. Binding sites for endothelin-1 in rat tissues: An autoradiographic study. J Cardiovasc Pharmacol. 1989; 13 (Suppl. 5): 5153–4.

    Google Scholar 

  17. Grisoni E, De Augustin JC, Kalhan SC. Vasoactive intestinal polypeptide causes relaxation of the pyloric sphincter in the rabbit. J Pediatr Surg. 1993; 28: 1117–20.

    Article  PubMed  CAS  Google Scholar 

  18. Krowicki ZK, Hornby PJ. Hindbrain neuroactive substances controlling gastrointestinal function. In: Gaginella TS, ed. Regulatory Mechanisms in Gastrointestinal Function. Boca Raton: CRC Press; 1995: 277–319.

    Google Scholar 

  19. Fuxe K, Andbjer B, Kalia M, Agnati LF. Centrally administered endothelin-1 produces apnoea in the α-chloralose-anaesthetized male rat. Acta Physiol Scand. 1989; 137: 157–8.

    Article  PubMed  CAS  Google Scholar 

  20. Krowicki ZK, Hornby PJ. Opposing gastric motor responses to TRH and substance P on their microinjection into nucleus raphe obscurus of rats. Am J Physiol. 1993; 265: G819–30.

    PubMed  CAS  Google Scholar 

  21. Krowicki ZK, Hornby PJ. Serotonin and thyrotropin-releasing hormone do not augment their effects on gastric motility on their microinjection into the nucleus raphe obscurus of the rat. J Pharmacol Exp Ther. 1995; 273: 499–508.

    PubMed  CAS  Google Scholar 

  22. MacCumber MW, Ross CA, Snyder SH. Endothelin in brain: Receptors, mitogenesis, and biosynthesis in glial cells. Proc Natl Acad Sci USA. 1990; 87: 2359–63.

    Article  PubMed  CAS  Google Scholar 

  23. Riley CR, Jones CR, Pelton JT, Miller RC. Binding of [125I]-endothelin-1 to rat cerebellar homogenates and its interactions with some analogues. Br J Pharmacol. 1990; 101: 319–24.

    Article  Google Scholar 

  24. Schvartz I, Ittoop 0, Hazum E. Identification of a single binding protein for endothelin-1 and endothelin-3 in bovine cerebellum membranes. Endocrinology. 1991; 128: 126–30.

    Article  PubMed  CAS  Google Scholar 

  25. Crawford MLA, Riley CR, Young JM. Characteristics of endothelin-1 and endothelin-3 stimulation of phosphoinositide breakdown differ between regions of guinea-pig and rat brain. Naunyn-Schmiedeberg’s Arch Pharmacol. 1990; 341: 268–71.

    Article  CAS  Google Scholar 

  26. Kuwaki T, Koshiya N, Takahashi H, Terui N, Kumada M. Modulatory effects of rat endothelin on central cardiovascular control in rats. Jpn J Physiol. l990;40:97–116.

    Article  PubMed  CAS  Google Scholar 

  27. Ouchi Y, Kim S, Souza AC et al. Central effect of endothelin on blood pressure of conscious rats. Am J Physiol. 1989;256:H1747–51.

    PubMed  CAS  Google Scholar 

  28. Asano T, Ikegaki I, Satoh SI et al. Endothelin: A potential modulator of cerebral vasospasm. Eur J Pharmacol. 1990; 190: 365–72.

    Article  PubMed  CAS  Google Scholar 

  29. Macrae IM, Robinson M, McAuley M, Reid J, McCulloch J. Effects of intracisternal endothelin-1 injection on blood flow to the lower brain stem. Eur J Pharmacol. 1991; 203: 85–91.

    Article  PubMed  CAS  Google Scholar 

  30. Hashim MA, Tadepalli AS. Hemodynamic responses evoked by endothelin from central cardiovascular neural substrates. Am J Physiol. 1992;262:H1–H9.

    PubMed  CAS  Google Scholar 

  31. Patino R, Ibarra J, Molino A, Fernandez-Durango R, Moya J, Fernandez-Cruz A. Increased plasma endothelin in diabetes: An atherosclerosis marker? Diabetologia. 1994; 37: 333–4.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology and Experimental Therapeutics, Louisiana State University Medical Center, New Orleans, LA, 70112, USA

    Z. K. Krowicki & P. J. Hornby

Authors
  1. Z. K. Krowicki
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  2. P. J. Hornby
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Editors and Affiliations

  1. School of Pharmacy, University of Wisconsin, Madison, WI, USA

    Timothy S. Gaginella

  2. First Department of Medicine, University Medical School of Pécs, Pécs, Hungary

    Gyula Mózsik

  3. Department of Biomedical Sciences, Sheffield Hallam University, Sheffield, UK

    K. D. Rainsford

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© 1997 Springer Science+Business Media Dordrecht

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Krowicki, Z.K., Hornby, P.J. (1997). Gastric Motor Effects of Endothelins in the Lower Brainstem of the Rat. In: Gaginella, T.S., Mózsik, G., Rainsford, K.D. (eds) Biochemical Pharmacology as an Approach to Gastrointestinal Disorders. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5390-4_11

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  • DOI: https://doi.org/10.1007/978-94-011-5390-4_11

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6267-1

  • Online ISBN: 978-94-011-5390-4

  • eBook Packages: Springer Book Archive

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