Abstract
The adsorption of nucleic acid probes (NAP) onto aminated silica wafers has been investigated. This study aims to determine the driving forces of the binding reaction. Aminopropylsilane modified silica surfaces were characterized by contact angle measurements, XPS, ellipsometry and AFM. The adsorbed amount of oligonucleotides was estimated by radioactive counting. Adsorption kinetic of the process revealed a high affinity between NAP and aminated surfaces despite a low yield of adsorption. Varying adsorption conditions, namely oligonucleotides initial concentration, pH and ionic strength, we have demonstrated that two driving forces are controlling the adsorption process: electrostatic interactions and dehydration effect.
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© 1998 Springer Science+Business Media Dordrecht
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Balladur, V., Mandrand, B., Theretz, A. (1998). Physico Chemical Studies of the Adsorption Process Between Aminated Silica Wafers and Oligonucleotides.. In: Coleman, A.W. (eds) Molecular Recognition and Inclusion. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5288-4_33
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DOI: https://doi.org/10.1007/978-94-011-5288-4_33
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6226-8
Online ISBN: 978-94-011-5288-4
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