Abstract
Porous silicon is generated in five different silicon types of <111>-orientation and the influence of dopant type and dopant concentration with respect to ability to serve as an enzyme activated biocatalytic substrate. Glucose oxidase was immobilised onto the porous surfaces and a maximum increase in catalytic efficiency of 350 times was demonstrated. The influence of the depth of the porous layer was investigated, showing that an average porous depth of no more than 5–10 μm is optimal for the parallel channel structured micro enzyme reactors developed by our group.
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M. U. Kopp, A. J. de Mello and A. Manz, Chemical amplification: Continuous-flow PCR on a chip, Science 280 (1998) 1046–1048
T. Laurell, J. Drott and L. Rosengren, Silicon wafer integrated enzyme reactors, Biosensors & Bioelectronics, 10 (1995) 289–299
T. Laurell, J. Drott, L. Rosengren and K. Lindström, Enhanced enzyme activity in silicon integrated enzyme reactors utilizing porous silicon as the coupling matrix, Sensors and Actuators B 31 (1996) 161–166
J. Drott, K. Lindström, L. Rosengren and T. Laurell, Porous silicon as the carrier matrix in microstructured enzyme reactors yielding high enzyme activities, J. Micromech. Microeng., 17 (1997) 14–23
H. von Gallati, Aktivitätbestimmung von Peroxidase mit Hife des Trinder-Reagens, J. Clin. Chem. Clin. Biochem., 15 (1977) 699–703
J. Drott, L. Rosengren, K. Lindström and T. Laurell, Pore morphology influence on catalytic turn-over for enzyme activated porous silicon matrices, Thin Solid Films, 1998, in press
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© 1998 Springer Science+Business Media Dordrecht
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Drott, J., Bengtsson, M., Rosengren, L., Laurell, T. (1998). Optimisation of Porous Silicon Carrier Matrices Applied in Chip Based Micro Enzyme Reactors. In: Harrison, D.J., van den Berg, A. (eds) Micro Total Analysis Systems ’98. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5286-0_31
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DOI: https://doi.org/10.1007/978-94-011-5286-0_31
Publisher Name: Springer, Dordrecht
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