Skip to main content

Effect of Azospirillum inoculation on growth and N2 fixation of maize subjected to different levels of FYM using 15N-dilution method

  • Chapter
Nitrogen Fixation with Non-Legumes

Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 79))

Abstract

Using the 15N dilution method, a pot experiment was conducted to study the effect of Azospirillum inoculation under different levels of farmyard manure (0, 5, and 10 t/ha) on N2 fixation, growth and N yield of two maize cultivars. The percentage of nitrogen fixation (%Ndfa) by Azospirillum inoculation varied from 6% to 31% according to Azospirillum species and plant cultivars. Application of FYM at the rate of 5 t/ha decreased the nitrogen fixation in both cultivars and it was completely inhibited at a higher level (10 t/ha). Significant increases in the N yield were recorded in both cultivars irrespective of the FYM level (up to 10 t/ha). Such increases were mainly attributed to increases in shoot dry weights, as well as increases in the percentage of N derived mainly from the soil and fertilizer, not from N2 fixation. Azospirillum spp. showed a higher degree of host specificity, and the degree of specificity occurred at plant cultivar level.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Abd-El-Malek Y, Abd-El-Salam M A, Monib M, El-Hadidy T T 1961. Bacteriological and chemical changes resulting from addition of certain organic materials to calcareous soils of the Mediterranean coastal region. Bull. Inst. Desert, Egypt. 11, 31–40.

    Google Scholar 

  • Baldani V L D, Dobereiner J 1980. Host-plant specificity in the infection of cereals with Azospirillum spp. Soil Biol. Biochem., 12, 433–439.

    Article  Google Scholar 

  • Baldani V L D, Alvarez M A De B, Baldani J I, Dobereiner J 1986. Establishment of inoculated Azospirillum spp. in the rhizosphere and in roots of field grown wheat and sorghum. Plant Soil, 90, 35–46.

    Article  Google Scholar 

  • Bashan Y, Ream Y, Levanony H, Sade A 1989. Nonspecific responses in plant growth, yield and root colonization of noncereals crop plants to inoculation with Azospirillum brasilense Cd. Can. J. Bot., 67, 1317–1324.

    Article  Google Scholar 

  • Bright J W, Conn H J 1919. Influence of stable manure upon soil population. N.Y. Agric. Exp. Stat. Bull., 67.

    Google Scholar 

  • Caballero-Mellado J, Carcano Mantiel M G, Mascarua-Esparza M A 1992. Field inoculation of wheat (Triticum aestivum) with Azospirillum brasilense under temperate climate. Symbiosis, 13, 243–253.

    Google Scholar 

  • Cohran W G 1950. Estimation of bacterial densities by means of most probable number. Biometric., 6,105–116.

    Article  Google Scholar 

  • Dart P J 1986. Nitrogen fixation associated with non-legumes in agriculture. Plant Soil, 90, 303–334.

    Article  CAS  Google Scholar 

  • Day J M, Dobereiner J 1976. Physiological aspects of N2 fixation by Spirillum from Digitaria roots. Soil. Biol. Biochem., 8, 45–50.

    CAS  Google Scholar 

  • Da Silva P M, Tsai S M, Bonetti R 1993. Response to inoculation and N fertilization for increased yield and biological nitrogen fixation of common bean. Plant Soil, 152, 123–130.

    Article  Google Scholar 

  • Dobereiner J and Pedrosa F O 1987 In: Nitrogen Fixing Bacteria in Non-leguminous Crop Plants. Springer, Berlin, 1–155.

    Google Scholar 

  • El-Hadidy T M T 1960. Bacteriological and chemical changes due to addition of certain materials to Mediterranean calcareous soils. MSc thesis, Faculty of Agriculture, Cairo University, Egypt.

    Google Scholar 

  • El-Komy H M A 1992. Studies on the genus Azospirillum from the rhizosphere of maize and rice plants. PhD thesis, Institute of Agricultural Microbiology, Academic Science of Russia, Sanktbe-terburg.

    Google Scholar 

  • Fages J 1994. Azospirillum inoculants and field experiments. In: Okon Y, ed. Azospirillum Plant Association, 87–110.

    Google Scholar 

  • Fiedler R, Proksch G 1975. The determination of N-15 by emission and mass spectrometry in biological analysis. A review. Anal. Chem. Acta, 78, 1–62.

    Article  CAS  Google Scholar 

  • Fried M, Middelboe V 1977. Measurement of amount of nitrogen fixed by a legume crop. Plant Soil, 47, 713–715.

    Article  CAS  Google Scholar 

  • Garacia de Salamone Y 1993. MSc thesis, University of Buenos Aires.

    Google Scholar 

  • Hegazi N A, Vlassak K, Monib M 1979. Effect of amendments, moisture and temperature on acetylene reduction in Nile Delta soils. Plant Soil, 51, 27–37.

    Article  CAS  Google Scholar 

  • Hegazi N A, Monib M, Amer H A, Shokr E 1983. Response of maize plants to inoculation with Azospirilla and/or straw amendment in Egypt. Can. J. Microbiol., 29, 888–894.

    Article  Google Scholar 

  • Jagnow G 1990. Differences between cereal crop cultivars in root-associated nitrogen fixation, possible causes of variable yield response to seed inoculation. Plant Soil, 123, 255–259.

    Article  CAS  Google Scholar 

  • Mertens T, Hess D 1984. Yield increases in spring wheat inoculated with Azospirillium lipoferum under greenhouse and field conditions of a temperate region. Plant Soil, 82, 87–99.

    Article  Google Scholar 

  • Millet E, Feldman M 1986. Yield response of a common spring wheat cultivar to inoculation with Azospirillum brasilense at various levels of nitrogen fertilization. Plant Soil, 80, 255–259.

    Article  Google Scholar 

  • Okon Y, Kapulnik Y 1986. Development and function of Azospirillum-inoculated roots. Plant Soil, 90, 3–16.

    Article  CAS  Google Scholar 

  • Peoples M B, Herridge D F, Ladha J K 1995. An efficient source of nitrogen for sustainable agricultural production. In: Ladha J K, Peoples M B, eds. Management of Biological Nitrogen Fixation for the Development of More Productive and Sustainable Agricultural Systems. Kluwer, Dordrecht, 3–28.

    Chapter  Google Scholar 

  • Plazinski J, Rolfe G 1985. Analysis of the pectolytic activity of Rhizobium and Azospirillum strains isolated from Trifolium repens. J. Plant Physoil., 120, 181–187.

    Article  CAS  Google Scholar 

  • Reynders L, Vlassak K 1982. Use of Azospirillum brasilense as a biofertilizer in intensive wheat cropping. Plant Soil, 66, 217–223.

    Article  Google Scholar 

  • Rolfe B G, Gresshoff P M, Shine J 1980. Rapid screening for symbiotic mutants of Rhizobium and white clover. Plant Sci. Lett., 19, 277–284.

    Article  Google Scholar 

  • Roper M M, Halsall M 1986. Use of products of straw by N2-fixing populations of bacteria in three soils from wheat-growing areas. Aust. J. Agric. Res., 37, 1–9.

    Article  CAS  Google Scholar 

  • Roper M M, Ladha J K 1995. Biological N2 fixation by heterotrophic and phototrophic bacteria in association with straw. Plant Soil, 174, 221–224.

    Article  Google Scholar 

  • Roper M M, Smith N A 1991. Straw decomposition and nitrogenase activity by free-living microorganisms from soil: effect of pH and clay content. Soil Biol. Biochem., 23, 275–283.

    Article  CAS  Google Scholar 

  • Roper M M, Turpin J A, Tompson J P 1994. Nitrogenase activity by free-living bacteria in soil in a long-term tillage and stubble management experiment on a vertisol. Soil Biol. Biochem., 26, 1087–1091.

    Article  CAS  Google Scholar 

  • Winogradsky S 1924. Influence of organic matter upon soil population. Compt. Rend. Acad. Sci., 178, 1236 (cited in Waksman, S A 1931).

    Google Scholar 

  • Zambre M A, Konde B K, Sonar K R 1984. Effect of Azotobacter chrococcum and Azospirillum brasilense inoculation under graded levels of nitrogen on growth and yield of wheat. Plant Soil, 79, 61–67.

    Article  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

El-Komy, H.M.A., Moharram, T.M.M., Safwat, M.S.A. (1998). Effect of Azospirillum inoculation on growth and N2 fixation of maize subjected to different levels of FYM using 15N-dilution method. In: Malik, K.A., Mirza, M.S., Ladha, J.K. (eds) Nitrogen Fixation with Non-Legumes. Developments in Plant and Soil Sciences, vol 79. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5232-7_6

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-5232-7_6

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6202-2

  • Online ISBN: 978-94-011-5232-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics