Abstract
Three bacterial isolates were obtained from the roots of rice, wheat and kallar grass. These isolates fix nitrogen in pure culture as confirmed by acetylene reduction assay in semi-solid nitrogen-free growth media. Production of growth hormones by these isolates was confirmed by colorimetric method. The isolates from wheat (strain Wb-3) and rice (strain N-4) were identified as Azospirillum brasilense and A. lipoferum, respectively, on the basis of their morphology, carbon utilization pattern and biochemical tests. The isolate K-1 from kallar grass, previously identified as Azospirillum brasilense, was confirmed to belong to a newly named genus Azoarcus (Reinhold-Hurek et al., 1993) by using 16S rRNA-targeted oligonucleotide probes and some morphological and physiological characteristics.
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Hassan, U., Mirza, M.S., Mehnaz, S., Rasul, G., Malik, K.A. (1998). Isolation and identification of diazotrophic bacteria from rice, wheat and kallar grass. In: Malik, K.A., Mirza, M.S., Ladha, J.K. (eds) Nitrogen Fixation with Non-Legumes. Developments in Plant and Soil Sciences, vol 79. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5232-7_23
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DOI: https://doi.org/10.1007/978-94-011-5232-7_23
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