Abstract
We cloned three structural genes PKS1, SCD1 and THR1 encoding for major melanin biosynthetic enzymes and one regulatory gene CMR1 of Colletotrichum lagenarium. Among them, SCD1 and THR1 proteins were purified by heterologous expression system using E. coli and polyclonal antibodies against those proteins were prepared. Melanization of appressoria is developmentally regulated system; conidia, germ tubes and infection hyphae were not melanized during morphogenesis from conidia. The wild type strain 104-T of C. lagenarium shows temperature sensitivity for appressorium formation. Temporal transcriptional and translational patterns of melanin biosynthetic genes in appressorium-differentiating and non-differentiating conidia indicated that both transcriptional and post-transcriptional regulations were involved in the expression of melanin biosynthetic genes.
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Kubo, Y. et al. (1998). Expression and Regulation of Melanin Biosynthetic Genes during Appressorium Formation of Colletotrichum Lagenarium . In: Kohmoto, K., Yoder, O.C. (eds) Molecular Genetics of Host-Specific Toxins in Plant Disease. Developments in Plant Pathology, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5218-1_28
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DOI: https://doi.org/10.1007/978-94-011-5218-1_28
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