Abstract
A major goal of our research program is a molecular description of factors that regulate the Pyrenophora tritici-repentis/wheat interaction. This goal includes identification and characterization of the gene(s) involved in pathogenicity and host specificity and of the mechanisms of susceptibility and resistance. P. tritici-repentis, causal agent of tan spot of wheat, is pathogenic because it produces a host-selective toxin(s) (HSTs). Unlike other known HSTs, the major toxin(s) produced by this fungus is a protein. The protein nature of the toxin provided an experimental advantage to the cloning of a gene responsible for toxin biosynthesis and subsequently a direct molecular genetical analysis of the role of this HST in the development of tan spot of wheat. With the ToxA gene cloned, we have initiated studies to elucidate the site- and mode-of-action of this proteinaceous host-selective toxin. Our objectives toward this goal encompass a diversity of approaches including an immunological, biochemical, and genetical approach.
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Ciuffetti, L.M., Tuori, R.P., Gaventa, J.M. (1998). Cloning and Expression of the Toxa Gene in Pyrenophora Triticirepentis . In: Kohmoto, K., Yoder, O.C. (eds) Molecular Genetics of Host-Specific Toxins in Plant Disease. Developments in Plant Pathology, vol 13. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5218-1_20
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DOI: https://doi.org/10.1007/978-94-011-5218-1_20
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