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Matrix-Assisted Laser Desorption-Ionization (MALDI) Mass Spectrometry: Principles and Applications

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Selected Topics in Mass Spectrometry in the Biomolecular Sciences

Part of the book series: NATO ASI Series ((ASIC,volume 504))

Abstract

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is an analytical technique for fast and precise mass determination of biological molecules. Intact molecular ions are produced by short pulsed laser irradiation of the biomolecules which are enbedded in a matrix consisting of small highly absorbing organic molecules. Mass analysis is most often carried out in a linear or reflector time-of-flight mass spectrometer. The accessible mass range is > 500 000 Da, a mass accuracy of < 50 ppm for peptides < 2000 Da can be reached, the sample amounts required are 1 pmol or less. Proteins, glycoproteins, oligonucleotides and oligosacharides can be analyzed.

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Authors and Affiliations

  1. Institute for Physical and Theoretial Chemistry, Department of Instrumental Analytical Chemistry, University of Frankfurt, Theodor-Stern-Kai 7, 60590, Frankfurt, F.R. Germany

    M. Karas & U. Bahr

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  1. M. Karas
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  2. U. Bahr
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Editors and Affiliations

  1. Medical School, University of Texas, Houston, Texas, USA

    Richard M. Caprioli

  2. SESMA-C.N.R., Napoli, Italy

    Antonio Malorni

  3. Dipartimento di Chimica, Arcavacata di Rende, Università della Calabria, Italy

    Giovanni Sindona

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Karas, M., Bahr, U. (1997). Matrix-Assisted Laser Desorption-Ionization (MALDI) Mass Spectrometry: Principles and Applications. In: Caprioli, R.M., Malorni, A., Sindona, G. (eds) Selected Topics in Mass Spectrometry in the Biomolecular Sciences. NATO ASI Series, vol 504. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5165-8_3

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