Abstract
UDP-N-Acetylglucosamine βl, 4-galactosyltransferase (GalT) belongs to the large family of glycosyltransferases, and is responsible for the transfer of galactose to N-acetylglucosamine terminated glycoproteins and glycolipids. Previous research works have shown the association of GalT with various physiological alterations such as cancer (Gaudushon et al 1983; Davey et al 1986; Uemura et al 1988), rheumathoid arthritis (Axford et al 1987; Wilson et al 1993) and hepatic diseases (Kim et al 1972; Stibler et al 1991; Xin et al 1995).
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References
Axford J.S., Mackenzie, L., Lydyard, P.M., Hay, F.C, Isenberg. D.A and Roitt J.M. (1987) Reduced B-cell galactosyltransferase activity in rheumathoid arthritis, Lancet 2, 1486–1488.
Barker, R., Olsen, K.W., Shaper J.H. and Hill, R.L. (1972) Agarose derivatives of uridine diphosphate and N-Acetylglucosamine for the purification of a Galactosytransferase, J. Biol. Chem. 247, 7135–7147.
Brew, K., Vanaman, T.C. and Hill, R.L. (1968) The role of a-Lactalbumin and the A protein in Lactose synthetase: A unique mechanism for the control of a biological reaction, Proc. Natl. Acad. Sci. USA 59, 491–497.
Brodbeck, U., Denton, W.L., Tanahashi, N. and Ebner, K. E. (1967) The isolation and identification of the B Protein of Lactose Synthetase as α-Lactalbumin, J. Biol. Chem. 242, 1391–1397.
Keshvara, L.M., Newton, E.M., Good, A.H., Hindsgaul, O. and Palcic M.M. (1992) Enzyme-linked immunosorbent assays for the measurment of blood group A and B glycosyltransferase activities, Glycoconjugate J 9, 16–20.
Keusch, J., Lydyard. P.M., Isenberg, D.A. and Delves, P.J. (1995) β 1, 4-Galactosyltransferase activity in B cells detected using a simple ELISA-based assay, Glycobiology 5, 365–370.
Kim, Y.S., Perdomo, J., Whitehead, J.S. and Curtis, K.J. (1972) Glycosyltransferases in human blood: Study of serum galactosyltransferase and N-acetylgalactosaminyl-transferase in patients with liver diseases, J. din. invest. 51, 2033–2039.
Kobayashi, K., Akaike, T. and Usui, T. (1994) Synthesis of poly(N-Acetyl-β-lactosaminide-carrying Acrylamide):Chemical enzyme hybrid process. Methods in Enzymology 242, Neoglycoconjugates Part A: Synthesis, Lee, Y.C. and Lee, R.T. Academic Press, San Diego, 226-235.
Kobayashi, K., Kobayashi, A. and Akaike, T. (1994) Culture of hepatocytes on a lactose-carrying polystyrene via asialoglycoprotein receptor mediated interactions, Methods in Enzymology 247, Neoglycoconjugates Part B, Biomedical applications, Lee, Y.C. and Lee, R.T. Academic Press, San Diego, pp. 409-418.
Merkle, R. K and Cummings, R.D. (1987) Lectin affinity chromatography of glycopeptides. Methods Enzymol. 138, 232 259.
Gauduchon, P., Tillie, C., Guyonnet, C, Heron, J., Bar-Guilloux, E. and Le Talaer, J. (1983) Clinical Value of serum glycoprotein galactosyltransferase levels in different histological types of ovarian carcinoma, Cancer Research 43, 4491–4496.
Davey, R.D., Milliken, S.M., Harvie, R.M., Cahill, E.J., Morgan, L.J., Kerestes, Z. and Levi, J.A.(1986) Serum galactosyltransferase as a prognostic marker in patients with solid tumors, Cancer Research 46, 5973–5975.
Stibler, H.and Borg, S. (1991) Glycoprotein glycosyltransferase activities in serum in alcohol-abusing patients and healthy controls Scand. J. Clin. Lab. Invest, 51, 43–51.
Stult, C.L.M. and Macher, B.A. (1990) Measurement of β Galactosyltransferase activity in cell extacts with an ELISA-based assay, Arch. Biochem. Biophys. 280, 20–26.
Taki, T., Nishikawa, S., Ishii, K.and Handa, S. (1990) A simple and specific assay of glycosyltransferase and glycosidase activities by an enzyme-linked immunosorbent assay method, and its application to assay of galactosyltransferase activity in sera from patients with cancer, J. Biochem. 107, 493–498.
Uemura, M., Winant, R.C.and Brandt, A.E. (1988) Immunoassay of serum galactosyltransferase isoenzyme 2 in cancer patients and control subjects. Cancer Research 48, 5335–5341.
Wilson, J. B. H., Platt, F.M., Isenberg, D.A. and Rademacher, T.W. (1993) Aberrant control of galactosyltransferase in peripheral B lymphocytes and Epstein-Barr virus transformed B lymphoblasts from patients with rheumatoid arthritis, J. Rheumathol 20, 1282–1287.
Xin, Y., Lasker J.M.and Lieber, C.S.(1995) Serum carbohydrate-deficient transferrin: Mechanism of increase after chronic alcohol intake, Hepatology 22, 1462–1468.
Zatta, P.F., Nyame K., Cormier, M.J., Mattox, S.A., Smith, D.F. and Cummings, R.D (1991) A solid phase assay for β l, 4-galactosyltransferase activity in human serum using recombinant aequorin, Anal. Biochem. 194, 185–191.
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Oubihi, M., Kitajima, K., Kobayashi, K., Adachi, T., Aoki, N., Matsuda, T. (1998). Development of an Elisa Based Assay for Measuring Glycosyltransferase Activity Using Synthetic Glycopolymers. In: Nagai, K., Wachi, M. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 9. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5161-0_30
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