Abstract
We developed a non-radioisotopic assay to detect telomerase activity in serum-free mouse embryo cells (SFME cells). Using this assay, telomerase regulation mechanism in differentiated SFME cells was investigated SFME cells treated with 10% serum or with 10 ng/ml of TGF- β showed astrocytes-like phenotypes and lowered telomerase activity. These results indicate that telomerase activity is down-regulated with the differentiation of SFME cells into astrocytes.
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Katakura, Y. et al. (1998). Telomerase regulation in differentiated serum-free mouse embryo cells. In: Nagai, K., Wachi, M. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 9. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5161-0_3
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DOI: https://doi.org/10.1007/978-94-011-5161-0_3
Publisher Name: Springer, Dordrecht
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