Summary
In order to study the antitumoral effect of Selaginella tamariscina extracts, the cytotoxicities to human histiocytic lymphoma(U937) and lymphocyte were measured by MTT method. The water extract of Selaginella tamariscina was partitioned into chloroform (CHC13), ethylacetate (EtOAc), n-butanol (BuOH) and water (H2O), successively. CHC13, EtOAc and BuOH fractions of Selaginella tamariscina had the cytotoxicity to the U937 cells but no effect on the lymphocyte under the same conditions. The expression of p53 tumor suppressor gene was evaluated by nothern blotting method. The increased expression of p53 was only induced by water fraction but CHCI3, EtOAc and BuOH fractions. To confirm the correlation between role of p53 and cell cycle phase for sensitivity of Selaginella tamariscina, the human colon carcinoma cell line HT-29 and the human ovarian cancer cell line A2780 were treated for 24h with 50 μg/ml of each fraction. In 2780 cells, the water fraction significantly showed the Gl arrest compared with the other fractions. These results suggest that the antitumoral effect of water fraction is related to p53 and Gl progression.
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References
Chang, C. J., Ashendel, C. L., Chan, T., Geahlen, R. L., and McLaughlin, J.L. (1990) Discovery of national antitumor drugs: new strategies, J. Nat.. Prod. 53, 23–25.
Lee, I. R, Song, J. Y., and Lee, Y. S. (1992) Cytotoxicity of Folkloric Medicine in Murine and Human Cancer Cells, Kor. J. Pharmacogn 23, 132–134.
Miller, C. and Koenffler, H.P. (1993) p53 mutations in human cancer Leukemia 7/suppl. 2. 18–20.
Rhee, I. J. and Park, S. H.(1994) Effect of selaginella tamariscina on U937 cytotoxicity, J. Korean Soc. Food Nutr. 23, 799–804.
Carmichael, J., DeGraff, A. F., Gazder, J. D., Minna, J. D., and Mitchell J.B. (1987) Evaluatio n of a tetrazolium-based semiautomatic colorimetric assay; Assessment of radiosensitivity, Cancer Res. 47, 943–945.
Carmichael, J., DeGraff, A. F., Gazder, J. D., Minna, J. D., and Mitchell J. B. (1987) Evaluatio n of a tetrazolium-based semiautomatic colorimetric assay; Assessment of radiosensitivity, Cancer Res. 47, 935–937.
Park, J. G., Kramer, B. S., Steinberg, S. M., Carmichael, J., Collins J. M., Minna, J. D., and Gaxder, A. F. (1987) Chemosensitivity testing of human coloretal carcinoma cell lines using a tetrazolium-based colorimetric assay, Cancer Res., 47, 5875–5878.
Mosman, T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assay, J. Immunol Methodes., 65, 55–57.
Maluish A. E. and Strong D. M.: Lymphocyte proliferation, In Rose, F. H. and Fahey J. L.(eds.) (1986), Mannual of clinical laboratory immunology (3rd.ed.), American Society for Microbiology, Washington, D.C., pp 274
Kataoka, S., Naito, M., Tomida, A., and Tsuruo, T. (1994) Resistance to antitumor agent induced apoptosis in a mutant of human myeloid leukemia U937 cells, Exp. Cell Res., 215, 199–204.
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Lee, I.S., Kim, S.U., Lee, Y.S., Park, S.H., Rhee, I.J. (1998). Molecular Mechanisms of Selaginella Tamariscina for Antineoplastic Activity in Human Leukemia Cell Line U937. In: Nagai, K., Wachi, M. (eds) Animal Cell Technology: Basic & Applied Aspects. Animal Cell Technology: Basic & Applied Aspects, vol 9. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5161-0_15
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DOI: https://doi.org/10.1007/978-94-011-5161-0_15
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