Abstract
The AFM [1–4] allows to control small micromanufactured tips with a precision of fractions of Ångströms at a force sensitivity of piconewtons. It is therefore natural to combine nanotechnology and life sciences to investigate the mechanical properties of individual biomolecules [5–12]. Here we report recordings of force extension curves of polysaccharides and polyaminoacids.
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References
Binnig, G. and Rohrer, H., (1987) Scanning Tunneling Microscopy - from birth to adolescence, Rev. Mod. Phys. 59 615–625.
Alexander, S., et al., (1989) An atomic-resolution atomic-force microsope implemented using an optical lever, J. Appl. Phys. 65 (1), 164–167.
Binnig, G., Quate, C.F., and Gerber, C., (1986) Atomic force microscope, Phys. Rev. Lett. 56 930.
Goodman, F.O. and Garcia, N., (1991) Roles of the attractive and repulsive forces in atomic-force microscopy, Phys. Rev. B (Condensed Matter) 43 (6), 4728–4731.
Rief, M., et al., (1997) Single molecule force spectroscopy on polysaccharides by AFM, Science Feb
Rief, M., et al., (1997) Reversible Unfolding of Individual Titin Immunoglobulin Domains by AFM, Science 276 1109–1112.
Florin, E.-L., Moy, V.T., and Gaub, H.E., (1994) Adhesive forces between individual ligand-receptor pairs., Science 264 415–417.
Moy, V.T., Florin, E.L., and Gaub, H.G., (1994) Intermolecular forces and energies between ligands and receptors, Science 266 257–259.
Dammer, U., et al., (1995) Specific antigen/antibody interactions observed by atomic force microscopy, Biophys. J. 70 (5), 2437–2441.
Hinterdorfer, P., et al., (1996) Detection and localization of individual antibody-antigen recognition events by atomic force microscopy, Proc. Natl. Acad. Sci. USA 93 3477–3481.
Lee, G.U., Chris, L.A., and Colton, R.J., (1994) Direct measurement of the forces between complementary strands of DNA, Science 266 771–773.
Radmacher, M., et al., (1994) Direct observation of enzyme activity with the atomic force microscope, Science 265 1577–1579.
Smith, S.B., Cui, Y., and Bustamante, C., (1996) Overstretching B-DNA: the elastic response of individual double-stranded and single-stranded DNA molecules, Science 271 795–798.
Li, H., et al., (1997) Single molecule force spectroscopy on Xanthan by AFM, Adv. Ma. in press
Florin, E.L., et al., (1995) Sensing specific molecular interactions with the atomic force microscope, Biosensors and Bioelectronics 10 (9–10), 895–901.
Davidson, B. and Fasman, G.D., (1967) The conformational transitions of uncharged poly-L-lysine., Biochemistry 6 (6), 1616–1629.
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© 1998 Springer Science+Business Media Dordrecht
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Rief, M., Schulz-Vanheyden, P., Gaub, H.E. (1998). Single Molecule Force Spectroscopy by AFM Reveals Details of Polymer Structure. In: García, N., Nieto-Vesperinas, M., Rohrer, H. (eds) Nanoscale Science and Technology. NATO ASI Series, vol 348. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5024-8_5
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DOI: https://doi.org/10.1007/978-94-011-5024-8_5
Publisher Name: Springer, Dordrecht
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