Abstract
Molecular approaches are particularly useful for measuring genetic diversity and were applied to samples of central stonerollers obtained from sites along tributaries to the Great Miami River in Ohio. We used Random Amplified Polymorphic DNA (RAPD) analysis to assess the level of genetic diversity within and among these populations. RAPD analysis generates genetic profiles that were used to develop indices of genetic similarity. The RAPD method provides a cost effective means of generating an arbitrary sample of anonymous loci across the genome and generate a virtually unlimited set of loci for use in genetic analysis in the absence of specific sequence information. These attributes make RAPDs well suited for use in evaluating the diversity and assessing the potential vulnerability to exposure of populations across multiple spatial scales. The results demonstrate that a significant amount of structuring exists among populations analyzed to date and that a trend exists towards genetic diversity being an inverse function of site distance from the main stem as well as a being directly related to stream order. This indicates that populations farthest from main conduits or in lower order streams, and thereby most isolated, may be the most vulnerable populations to stressor exposure. It is hoped that information pertaining to genetic diversity, when integrated with other metrics of resource condition, will aid in making scientifically grounded decisions on resource management that enhance the probability of population survival and preserve natural evolutionary processes.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Altendorf, F.W., Leary, R.F.: 1986, Conservation Biology, Sinauer Associates, Sunderland, p 57.
Angermeier, P.L., Karr, J.R.: 1994, BioScience 44, 690.
Avise, J.C., Arnold, J., Ball, R.M., Bermingham, E., Lamb, T., Neigel, J.E., Reeb, C.A., Saunders, N.C.: 1987, Annual Review of Ecology and Systematics 18, 489.
Caetano-Anolles, G., Bassam, B.J., Gresshoff, P.M.: 1991, Bio/Technology 9, 553.
Caswell, H.: 1976, Ecol. Monogr. 46, 327.
Dobzhansky, T.: 1950, Sci. Amer. 182(1), 32.
Lattier, D.L., Gordon, D.A., Silbiger, R.N., McCormick, F., Smith, M.K.: 1996, Techniques in Aquatic Toxicology, Lewis Publishers, Boca Raton, p. 569.
Leberg, P.L.: 1990, Journal of Fish Biology 37, 193.
Lynch, M.: 1988, Mol. Biol. Evol. 5(5), 584.
Lynch, M.: 1990, Molecular. Biology. &. Evolution 7, 478.
Lynch, M.: 1991, DNA Fingerprinting: Approaches and Applications, Birkhauser Verlag, Switzerland.
Lynch, M., Milligan, B.G.: 1994, Molecular. Ecology. 3, 91.
MacArthur, R.H., Wilson, E.O.: 1967, The Theory of Island Biogeography, Princeton University Press, Princeton.
MacLean, J.A., Evans D.O.: 1981, Can J. Fish. Aquas. Sci. 38, 1889.
Nehlsen, W., Williams, J.E., Lichatowich, J.A.: 1991, Fisheries 16, 4.
Ohio Environmental Protection Agency: 1987, Biological Criteria for the Protection of Aquatic Life, Vol II, Division of Water Quality Monitoring and Assessment, Surface Water section, Columbus.
Omemik, J.M.: 1995, Biological Assessment and Criteria: Tools for water resource planning and decision making, Lewis Publishers, Boca Raton, p. 49.
Patton, J.L., Smith, M.F.: 1990, The Evolutionary dynamics of the pocket gopher Thomomys bottae, emphasis on California populations, University of California Press, Berkley.
Quatro, J.M., Vrijenhoek, R.C.: 1989, Science 245, 976.
Root, T.L., Schneider, S.H.: 1995, Science 269, 334.
Sanjayan, M.A., Crooks, K.: 1996, Nature 381, 566.
Sardelli, A.D.: 1993, Amplifications 1.
Smith, T.B. and Wayne, R.K.: 1996, Molecular Genetic Approaches in Conservation, Oxford University Press, New York.
Soulé, M.E. and Wilcox, B.A.: 1980, Conservation Biology: an evolutionary-ecological perspective, Sinauer Associates, Sunderland.
Soulé, M.E.: 1986, Conservation Biology: the science of scarcity and diversity, Sinauer, Sunderland.
Soulé, M.E., Zegers, G.P.: 1996, Journal of Heredity 87, 341.
Stone, R.: 1995, Science 269, 316.
United States Environmental Protection Agency: Klemm, D.J. and I Lazorchak, J.M.: 1993, Environmental Monitoring and Assessment Program. Surface Waters and Region 3 Regional Environmental Monitoring and Assessment Program. 1993 Pilot Field Operation and Methods Manual. Streams, USEPA, ORD, EMSL-Ci, Washington, D.C.
Welsh, J., McClelland, M.: 1990, Nucl. Acids Res. 18, 7213.
Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V.: 1990, Nucleic. Acids. Research. 18, 6531.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Silbiger, R.N. et al. (1998). Preliminary Studies on the Population Genetics of the Central Stoneroller (Campostoma anomalum) from the Great Miami River Basin, Ohio. In: Sandhu, S., Jackson, L., Austin, K., Hyland, J., Melzian, B., Summers, K. (eds) Monitoring Ecological Condition at Regional Scales. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4976-1_37
Download citation
DOI: https://doi.org/10.1007/978-94-011-4976-1_37
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6089-9
Online ISBN: 978-94-011-4976-1
eBook Packages: Springer Book Archive