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Evolution and Impact of Transposable Elements pp 39–48Cite as

Structural analysis of Drosophila subobscura gypsy elements (gypsyDs)

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Part of the book series: Contemporary Issues in Genetics and Evolution ((CIGE,volume 6))

Abstract

The study of gypsy elements in Drosophila subobscura (gypsyDs) indicated that they are transcriptionally active and mobile. From the comparative analysis of a complete gypsyDs element with the canonical gypsy sequence from D. melanogaster (gypsyDm) it can be deduced that while the whole structure is maintained, the gypsyDs ORF3 encodes a non-functional Env protein. the PCR amplification and sequencing of the ORF3 from different laboratory strains and H271 clones show that all gypsyDs sequences studied have frame-shifting mutations in this region. These results support that gypsyDs elements lack functional Env proteins and consequently they lack infective ability. In this way, it can be proposed that gypsyDs elements are degenerate forms of insect retroviruses. Heterogeneous results have been obtained in the study of the presence of gypsyDm sequences in different D. subobscura strains indicating that these sequences are unstable in this species.

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Authors and Affiliations

  1. Departamento de Genética, Facultad de Ciencias Biológicas, Universidad de Valencia, Dr. Moliner 50 Burjassot, 46100, Valencia, Spain

    T. M. Alberola, L. Bori & R. de Frutos

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  1. T. M. Alberola
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  2. L. Bori
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  3. R. de Frutos
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Pierre Capy

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© 1997 Springer Science+Business Media Dordrecht

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Alberola, T.M., Bori, L., de Frutos, R. (1997). Structural analysis of Drosophila subobscura gypsy elements (gypsyDs). In: Capy, P. (eds) Evolution and Impact of Transposable Elements. Contemporary Issues in Genetics and Evolution, vol 6. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4898-6_4

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  • DOI: https://doi.org/10.1007/978-94-011-4898-6_4

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6054-7

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