Abstract
The simplest way to detect the presence of a lectin in a biological material is to prepare a water or buffer extract from this material and to examine its ability to agglutinate erythrocytes or to precipitate a polysaccharide or glycoprotein. If a positive result is obtained, it is essential to show that agglutination or precipitation is specifically inhibited by mono- or oligosaccharides, i.e. it is sugar specific (Fig. 3.1).
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© 1989 Nathan Sharon and Halina Lis
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Sharon, N., Lis, H. (1989). Occurrence and isolation. In: Lectins. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4846-7_3
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DOI: https://doi.org/10.1007/978-94-011-4846-7_3
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6029-5
Online ISBN: 978-94-011-4846-7
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