Abstract
In order to characterize promoters for the nuclear-encoded plastid RNA polymerase (NEP), we developed a novel in vitro transcription system. The assay is based on extracts from plants lacking the plastid-encoded RNA polymerase (PEP) activity due to targeted deletion of the PEP α subunit gene. We report here the characterization of PaccD-129, the promoter for accD, encoding a subunit of the acetyl-CoA carboxylase. Dissection of a ~0.8 kb fragment determined that the accD promoter is contained within a 19-bp fragment (-17/+2).
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© 1999 Springer Science+Business Media Dordrecht
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Liere, K., Maliga, P. (1999). Novel in Vitro Transcription Assay Indicates that the ACCD Nep Promoter is Contained in a 19 BP Fragment. In: Argyroudi-Akoyunoglou, J.H., Senger, H. (eds) The Chloroplast: From Molecular Biology to Biotechnology. NATO Science Series, vol 64. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4788-0_11
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DOI: https://doi.org/10.1007/978-94-011-4788-0_11
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